The N uptake-associated physiological processes at late growth stage in wheat (Triticum aestivum) under N deprivation combined with deficit irrigation condition.
The N uptake-associated physiological processes at late growth stage in wheat (Triticum aestivum) under N deprivation combined with deficit irrigation condition.
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DOI：
10.1016/j.plaphy.2021.04.023
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发表时间：
2021-05
期刊：
影响因子：
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通讯作者：
Yanyang Zhang;Yingjia Zhao;Liyong Sun;P. Han;Xinyang Bai;Ruize Lin;K. Xiao
中科院分区：
文献类型：
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作者：
Yanyang Zhang;Yingjia Zhao;Liyong Sun;P. Han;Xinyang Bai;Ruize Lin;K. Xiao
阐明植物氮吸收的生理机制有利于培育高氮利用效率（NUE）作物品种。在本研究中，我们使用两个对比的 NUE 品种研究了缺氮和亏缺灌溉下小麦的生长和氮吸收相关过程。与充足氮（SN）相比，缺氮（DN）处理降低了两个品种（高NUE施农086和氮缺乏敏感型济麦585）的植物生物量、氮积累和产量，表明氮缺乏对植物生长和氮吸收产生负调节。施农086在生长和氮吸收相关性状方面优于济麦585，因为整个土壤剖面根系生物量得到改善，这与土层速效氮含量的下降相一致。这些结果表明，改善的根系结构（RAS）增强了植物在缺氮和缺水条件下对土壤氮的获取，有助于植物氮的吸收和产量形成能力。转录组研究表明，缺氮的石农086植物中存在许多基因差异表达（DE），这些基因涉及复杂的生化途径的调节。这些结果表明，高NUE植物中RAS和N吸收的改变是在众多DE基因的调控下完成的。TaWRKY20是ZFP转录因子家族中的一个基因，其功能特征在于介导植物N吸收的作用。由于其对两个硝酸盐转运蛋白基因 TaNRT2.1 和 TaNRT2.2 的调节，其过表达可改善 DN 下植物的生长和氮吸收。我们的研究提供了关于氮缺乏下小麦高氮肥利用机制的见解。
Elucidating physiological mechanisms underlying the plant N uptake benefits breeding of high N use efficiency (NUE) crop cultivars. In this study, we investigated the growth and N uptake-associated processes in wheat under N deprivation and deficit irrigation, using two contrasting NUE cultivars. Compared with sufficient-N (SN), deficient-N (DN) treatment reduced plant biomass, N accumulation, and yields in two cultivars (high NUE Shinong 086 and N deprivation-sensitive Jimai 585), suggesting that N deprivation negatively regulates plant growth and N uptake. Shinong 086 was better on growth and N uptake-associated traits than Jimai 585 due to the improved root biomass across soil profile, which was consistent with the decrease of available N contents in soil layers. These results suggested that the improved root system architecture (RAS) enhances plant acquirement for soil N under N- and water-deprivation condition, contributing to the plant N uptake and yield formation capacities. Transcriptome investigation revealed that numerous genes were differentially expressed (DE) in the N-deprived Shinong 086 plants, which involve the regulation of complicate biochemical pathways. These results suggested that the modified RAS and N uptake in high NUE plants are accomplished underlying the regulation of numerous DE genes.TaWRKY20, a gene in ZFP transcription factor family, was functionally characterized for the role in mediating plant N uptake. Overexpression of it conferred plants improved growth and N uptake under DN due to its regulation onTaNRT2.1andTaNRT2.2, two nitrate transporter genes. Our investigation provides insights in high NUE mechanisms in wheat under N deprivation.