Investigation of Lipid Metabolism by a New Structured Lipid with Medium- and Long-Chain Triacylglycerols from Cinnamomum camphora Seed Oil in Healthy C57BL/6J Mice
Investigation of Lipid Metabolism by a New Structured Lipid with Medium- and Long-Chain Triacylglycerols from Cinnamomum camphora Seed Oil in Healthy C57BL/6J Mice
复制标题
香樟籽油中长链三酰甘油的新型结构脂质对健康 C57BL/6J 小鼠脂质代谢的研究
DOI：
10.1021/acs.jafc.7b05659
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发表时间：
2018-02-28
影响因子：
6.1
通讯作者：
Deng, Ze-Yuan
中科院分区：
文献类型：
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作者：
Hu, Jiang-Ning;Shen, Jin-Rong;Deng, Ze-Yuan
本研究以山茶油(CO)和樟子油(CCSO)为原料，通过酶促酯交换反应合成了一种含有中长链三酰甘油(MLCTs)的新型结构油脂。同时，通过观察C57BL/6J小鼠体内脂肪动员相关酶的变化，探讨结构化脂肪的减肥作用。结果表明，合成后的三酰基锗(TAG)主要为LACC/CLAC(12.6+/-0.46%)、LACO/LCL(21.7+/-0.76%)、CCO/LaCL(14.2+/-0.55%)、COO/OCO(10.8+/-0.43%)和OOO(18.6+/-0.64%)。通过第二阶段分子蒸馏，酯交换产物(MLCT)的纯度达到95.6%。随后，应用雄性C57BL/6J小鼠研究了新型MLCT结构化脂质是否具有预防肥胖形成的作用。用不同饲料喂养6周后，MLCTs组小鼠体重和脂肪沉积减少，血浆甘油三酯(TG)(0.89+/-0.16 mm o l/L)、总胆固醇(TC)(4.03+/-0.08 mm o l/L)和肝脂(382+/-34.2 mg/只)较对照2组分别降低28.8%、16.0%和30.5%。随之而来的是MLCT组大鼠粪便中脂质含量升高(113%)以及与脂质动员相关的酶水平，包括环磷酸腺苷(CAMP)、蛋白激酶A(PKA)、激素敏感脂肪酶(HSL)和脂肪甘油三酯脂肪酶(ATGL)。结果表明，MLCT可减少C57BL/6J小鼠体内脂肪沉积，其机制可能与调节脂肪动员相关酶有关。
In the present study, a new structured lipid with medium- and long-chain triacylglycerols (MLCTs) was synthesized from camellia oil (CO) and Cinnamomum camphora seed oil (CCSO) by enzymatic interesterification. Meanwhile, the antiobesity effects of structured lipid were investigated through observing the changes of enzymes related to lipid mobilization in healthy C57BL/6J mice. Results showed that after synthesis, the major triacylgeride (TAG) species of intesterificated product changed to LaCC/CLaC (12.6 +/- 0.46%), LaCO/LCL (21.7 +/- 0.76%), CCO/LaCL (14.2 +/- 0.55%), COO/OCO (10.8 +/- 0.43%), and OOO (18.6 +/- 0.64%). Through second-stage molecular distillation, the purity of interesterified product (MLCT) achieved 95.6%. Later, male C57BL/6J mice were applied to study whether the new structured lipid with MLCT has the efficacy of preventing the formation of obesity or not. After feeding with different diets for 6 weeks, MLCTs could reduce body weight and fat deposition in adipose tissue, lower plasma triacylglycerols (TG) (0.89 +/- 0.16 mmol/L), plasma total cholesterol (TC) (4.03 +/- 0.08 mmol/L), and hepatic lipids (382 +/- 34.2 mg/mice) by 28.8%, 16.0%, and 30.5%, respectively, when compared to the control 2 group. This was also accompanied by increasing fecal lipids (113%) and the level of enzymes including cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), hormone-sensitive lipase (HSL), and adipose triglyceride lipase (ATGL) related to lipid mobilization in MLCT group. From the results, it can be concluded that MLCT reduced body fat deposition probably by modulating enzymes related to lipid mobilization in C57BL/6J mice.