Bioorthogonal SERS Nanotags as a Precision Theranostic Platform for in Vivo SERS Imaging and Cancer Photothermal Therapy
Bioorthogonal SERS Nanotags as a Precision Theranostic Platform for in Vivo SERS Imaging and Cancer Photothermal Therapy
复制标题
生物正交 SERS 纳米标签作为体内 SERS 成像和癌症光热治疗的精密治疗平台
DOI：
10.1021/acs.bioconjchem.0c00022
复制
发表时间：
2020-02-01
影响因子：
4.7
通讯作者：
Tang, Xinjing
中科院分区：
文献类型：
--
作者：
Wang, Jing;Liang, Duanwei;Tang, Xinjing
精确的检测和有效的治疗对于延长癌症患者的生命至关重要。表面增强拉曼散射（Sers）成像与光热治疗相结合被认为是一种精确有效的癌症治疗诊断策略。然而，文献中使用的拉曼报告分子通常在指纹区具有多个位移峰，这些位移峰与内源性生物分子的背景信号重叠。在此，我们制备了一种新的生物正交拉曼报告子和适体功能化Sers纳米标签。Sers纳米标签在2205 cm（-1）处的生物拉曼沉默区显示出强拉曼信号，并以高特异性识别MCF-7乳腺癌细胞进行拉曼成像。由于Sers纳米标签的优异光热能力，激光照射诱导了MCF-7细胞的严重毒性。在静脉内施用Sers纳米标签后，成功地实现了活体小鼠中的肿瘤拉曼光谱检测和绘图。进一步的体内抗肿瘤实验表明，适配子修饰的Sers纳米标签对激光照射后的肿瘤生长有明显的抑制作用，抑制率达99%，且具有良好的生物相容性。这些结果清楚地表明，Sers纳米标签可以作为一种新的和精确的治疗诊断平台，用于体内癌症诊断和光热治疗。
Precise detection and effective treatment are crucial to prolong cancer patients' lives. Surface-enhanced Raman scattering (SERS) imaging coupled with photothermal therapy has been considered a precise and effective strategy for cancer theranostics. Nevertheless, Raman reporters employed in the literature usually possessed multiple shift peaks in the fingerprint region, which are overlapped with background signals from endogenous biological molecules. Herein, we fabricated a new kind of bioorthogonal Raman reporter and aptamer functionalized SERS nanotags. The SERS nanotags demonstrated a strong Raman signal at 2205 cm(-1) in the biologically Raman-silent region and recognized MCF-7 breast cancer cells for Raman imaging with high specificity. Laser irradiation induced serious toxicity of MCF-7 cells due to the excellent photothermal capability of the SERS nanotags. After intravenous administration of the SERS nanotags, tumor Raman spectral detection and mapping in living mice were successfully achieved. Further in vivo antitumor experiments manifested that the aptamer-modified SERS nanotags significantly restrained tumor growth after laser irradiation with 99% inhibition rate and good biocompatibility. These results clearly revealed that the SERS nanotags could serve as a novel and precise theranostic platform for in vivo cancer diagnosis and photothermal therapy.