Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment.
Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment.
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布伦西斯脱硫孢菌 sp.
DOI：
10.1099/ijs.0.035238-0
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发表时间：
2013
影响因子：
2.8
通讯作者：
M. Labat
中科院分区：
文献类型：
--
作者：
Bruno Mayeux;M. Fardeau;Manon Bartoli;L. Casalot;A. Vinsot;M. Labat
从法国布雷-490m井孔的孔隙水中分离到一株新型厌氧、革兰氏阳性、芽胞形成、弯曲杆状、中温和硫酸盐还原细菌。该菌株命名为BSREI1(T)，生长温度为5℃至30℃(最适25℃)，pH为6至8(最适7℃)。它的生长不需要氯化钠，但能耐受高达1.5 %的氯化钠。采用硫酸盐、硫代硫酸盐和元素硫作为末端电子受体。菌株BSREI1(T)在硫酸盐存在下以巴豆酸盐、甲酸盐、乳酸、丙酮酸、果糖、甘油和酵母膏为电子供体。唯一的对苯二酚为MK-7。基因组DNA中G+C含量为43.3mol%。菌株BSREI1(T)的亲缘关系最近的模式菌株分别为乳链球菌(16SRRNA基因序列相似性为96.83 %)、子午链球菌属(96.31 %)和链球菌属(96.16 %)。根据系统发育和生理特性，BSREI1(T)菌株被认为是球孢霉属Desulfosporosinus burensis sp.的一个新种。模式菌株为BSREI1(T)( = DSM 24089(T) = JCM 17380(T))。
A novel anaerobic, gram-positive, spore-forming, curved rod-shaped, mesophilic and sulfate-reducing bacterium was isolated from pore water collected in a borehole at -490 m in Bure (France). This strain, designated BSREI1(T), grew at temperatures between 5 °C and 30 °C (optimum 25 °C) and at a pH between 6 and 8 (optimum 7). It did not require NaCl for growth, but tolerated it up to 1.5 % NaCl. Sulfate, thiosulfate and elemental sulfur were used as terminal electron acceptors. Strain BSREI1(T) used crotonate, formate, lactate, pyruvate, fructose, glycerol and yeast extract as electron donors in the presence of sulfate. The sole quinone was MK-7. The G+C content of the genomic DNA was 43.3 mol%. Strain BSREI1(T) had the type strains of Desulfosporosinus lacus (16S rRNA gene sequence similarity of 96.83 %), Desulfosporosinus meridiei (96.31 %) and Desulfosporosinus hippei (96.16 %) as its closest phylogenetic relatives. On the basis of phylogenetic and physiological properties, strain BSREI1(T) is proposed as a representative of a novel species of the genus Desulfosporosinus, Desulfosporosinus burensis sp. nov.; the type strain is BSREI1(T) ( = DSM 24089(T) = JCM 17380(T)).