Turnover and migration of meibomian gland cells in rats' eyelids
Turnover and migration of meibomian gland cells in rats' eyelids
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DOI：
10.1159/000055665
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发表时间：
2001-05-01
影响因子：
2.1
通讯作者：
Pe'er, J
中科院分区：
文献类型：
--
作者：
Olami, Y;Zajicek, G;Pe'er, J
目的：研究眉板腺细胞的运动速度和迁移过程。材料和方法：成年大鼠27只，体重约200g，单次注射Ci[H-3]-胸腺嘧啶核苷0.5mU/g体重。分别于注射后1h、3、6、9、12、15、18、21、28d各处死大鼠3只。眼球和眼皮被取出一块，用福尔马林固定，石蜡包埋，沿着瞳孔-视神经轴切成5微米厚的垂直切片。切片浸泡在液体放射自显影乳剂中，曝光3周，HE染色。使用眼睛微米栅格扫描左眼下眼皮中的眉肌腺。在每一次扫描中，每个腺泡的基底膜作为起始点。沿着整个腺体，每一个含有3个或更多颗粒的细胞核有两个参数：距基底膜的距离(Y)和颗粒数。结果：本研究首次证实了眉板腺细胞迁移的确切时间和方向。我们发现，细胞从基底膜迁移到腺泡中心的平均速度为0.62+/-0.11微米/天。细胞生成时间平均为4.1d。结论：眉板腺的干细胞位于每个腺泡的周缘。这些细胞以恒定的速度向腺泡中心迁移，并以皮脂物质的形式分泌。版权所有(C)2001 S.Karger AG，巴塞尔。
Purpose: The object of this study is to determine the velocity and migration course of meibomian gland cells. Materials and Methods: Twenty-seven adult rats weighing about 200 g were injected intraperitoneally with a single dose of 0.5 mu Ci [H-3]-thymidine/g body weight. Three rats were sacrificed 1 h after injection and 3 rats each on days 3, 6, 9, 12, 15, 18, 21 and 28 after injection. The eyes and eyelids were removed in one piece, fixed in formalin, embedded in paraffin and cut into 5-mum-thick vertical sections along the pupillary-optic nerve axis. The sections were dipped in liquid autoradiographic emulsion, exposed for 3 weeks, and stained with HE. Meibomian glands were scanned in the lower eyelid of the left eye using an ocular micrometer grid. In each scan the basement membrane of each acinus served as a point of origin. Along the entire gland two parameters were noted for each nucleus with three grains or more: the distance from the basement membrane (Y) and grain count. Results: This study demonstrates for the first time the exact timing and direction of meibomian gland cell migration. We have shown that the cells migrate from the basement membrane to the center of the acinus at an average rate of 0.62 +/- 0.11 mum/day. Cell generation time was an average of 4.1 days. Conclusions: The stem cells of meibomian glands lie at the circumference of each acinus. These cells migrate synchronously with a constant velocity towards the center of the acinus, and are secreted as sebaceous material. Copyright (C) 2001 S. Karger AG, Basel.