SsrA (tmRNA) acts as an antisense RNA to regulate Staphylococcus aureus pigment synthesis by base pairing with crtMN mRNA
SsrA (tmRNA) acts as an antisense RNA to regulate Staphylococcus aureus pigment synthesis by base pairing with crtMN mRNA
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SsrA (tmRNA) 作为反义 RNA，通过与 crtMN mRNA 碱基配对来调节金黄色葡萄球菌色素合成
DOI：
10.1016/j.febslet.2010.09.024
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发表时间：
2010-10-22
期刊：
影响因子：
3.5
通讯作者：
Yang, Guang
中科院分区：
文献类型：
--
作者：
Liu, Yu;Wu, Na;Yang, Guang
SsrA RNA（small stable RNA A），也称为tmRNA和10Sa RNA，通过其独特的结构同时具有tRNA和mRNA的功能。类胡萝卜素色素是人类病原菌金黄色葡萄球菌的表皮特征。在这里，我们发现，与ssrA缺失的突变株的色素增加。此外，ssrA除了具有已知的生物学功能外，还可以作为反义RNA发挥作用，而crtMN（编码色素合成的两种必需酶）被鉴定为ssrA的靶基因。进一步的研究表明ssrA可以与crtMN mRNA的RBS（核糖体结合位点）区域特异性碱基配对。我们的研究结果揭示了ssrA调控S.金黄色。(C)2010年欧洲生化学会联合会。Elsevier B.V.出版，保留所有权利。
SsrA RNA (small stable RNA A), also known as tmRNA and 10Sa RNA, functions both as tRNA and mRNA through its unique structure. The carotenoid pigment is the eponymous feature of human pathogen Staphylococcus aureus. Here, we found that the pigment of the mutant strain with ssrA deletion was increased. Furthermore, it was demonstrated that ssrA could act as an antisense RNA aside from its well-known biological function, and crtMN, encoding two essential enzymes for the pigment synthesis, was identified as target of ssrA. Further investigation showed ssrA could specifically base pair with the RBS (ribosomal binding site) region of the crtMN mRNA. Our results revealed a new mechanism by which ssrA regulated the biosynthesis of pigment in S. aureus. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.