ENHANCED EXPRESSION OF MUSCLE-SPECIFIC ACTIN IN GLOMERULONEPHRITIS
ENHANCED EXPRESSION OF MUSCLE-SPECIFIC ACTIN IN GLOMERULONEPHRITIS
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DOI：
10.1038/ki.1992.173
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发表时间：
1992-05-01
影响因子：
19.6
通讯作者：
JOHNSON, RJ
中科院分区：
文献类型：
--
作者：
ALPERS, CE;HUDKINS, KL;JOHNSON, RJ
大鼠肾小球系膜细胞损伤和增殖与"肌肉特异性"肌动蛋白表达增加有关。 我们使用两种针对"肌肉特异性"肌动蛋白的单克隆抗体（HHF-35，一种针对泛肌肉肌动蛋白的单克隆抗体和α-SM-1，一种针对α-平滑肌肌动蛋白的单克隆抗体）对甲基Carnoy固定的人肾活检进行了类似的免疫组织化学研究，这些肾活检证实了各种炎症性、增殖性和非增殖性肾小球疾病。大多数肾小球疾病与平滑肌肌动蛋白表达增加有关。 几乎无一例外地是没有显著细胞增殖的疾病环境。 在大鼠中，有一个相关性的诱导肌动蛋白表达与肾小球细胞增殖增加，如用单克隆抗体染色增殖细胞核抗原（PCNA）。 用白细胞共同抗原的抗体进行的双重免疫标记研究表明，绝大多数PCNA+增殖细胞是固有肾小球细胞，而不是浸润性白细胞。 这些研究表明，系膜细胞的表型变化发生在人类和实验性肾小球肾炎，并在固定的组织切片中可识别。这些研究还表明，系膜细胞损伤或活化和增殖的标志物，如"肌肉特异性"肌动蛋白的肾活检免疫染色，可能是有用的诊断和/或预后指标，在增生性或硬化性肾小球疾病。
Increased expression of "muscle-specific" actin can be correlated with mesangial cell injury and proliferation in the rat. We performed similar immunohistochemical studies using two monoclonal antibodies (MoAb) to "muscle-specific" actins (HHF-35, a MoAb to pan muscle actin and alpha-SM-1, a MoAb to alpha-smooth muscle actin) on methyl Carnoy's fixed human renal biopsies which demonstrated a wide variety of inflammatory, proliferative, and non-proliferative glomerular diseases. Most glomerular diseases were associated with increased "smooth-muscle" actin expression. Exceptions almost invariably were disease settings without prominent cellular proliferation. As in the rat, there was a correlation of induced actin expression with increased glomerular cell proliferation, as detected by staining with a MoAb to proliferating cell nuclear antigen (PCNA). Double immunolabeling studies with an antibody to the leukocyte common antigen showed the great majority of PCNA+ proliferating cells to be intrinsic glomerular cells rather than infiltrating leukocytes. These studies demonstrate that phenotypic changes of mesangial cells occur in both human and experimental glomerulonephritis, and are identifiable in fixed tissue sections. These studies also suggest that markers of mesangial cell injury or activation and proliferation, such as immunostaining of renal biopsies for "muscle-specific" actins, might be useful diagnostic and/or prognostic indicators in proliferative or sclerosing glomerular diseases.