Integrating multiple omics to identify common and specific molecular changes occurring in Arabidopsis under chronic nitrate and sulfate limitations
Integrating multiple omics to identify common and specific molecular changes occurring in Arabidopsis under chronic nitrate and sulfate limitations
复制标题
整合多个组学来剖析拟南芥 (L.) 在硝酸盐和硫酸盐慢性限制下发生的常见和特定分子变化。
DOI：
10.1093/jxb/eraa337
复制
发表时间：
2020-10-22
影响因子：
6.9
通讯作者：
Masclaux-Daubresse, Celine
中科院分区：
文献类型：
--
作者：
Luo, Jie;Have, Marien;Masclaux-Daubresse, Celine
植物对氮和硫有着根本的依赖性，当它们的供应不是最佳的时候，它们经常不得不科普长期的限制。本研究旨在表征慢性硝酸盐（低N）和慢性硫酸盐（低S）限制下拟南芥叶片中发生的代谢组学，蛋白质组学和转录组学变化，以比较它们的影响，确定相互联系，并研究适应策略。代谢物分析显示，低硫和低氮对碳水化合物和氨基酸积累的影响相反，而蛋白质组学数据显示，这两种处理导致分解代谢过程的增加，线粒体和胞质代谢的刺激，以及叶绿体代谢的减少。低氮和低硫条件下核糖体蛋白和翻译因子的丰度较低，与生长限制相对应。在转录水平上，低氮胁迫的主要特异性效应是增强防御和免疫基因的表达。慢性低S的主要影响是参与细胞分裂，DNA复制和细胞骨架的基因转录减少，自噬基因表达增加。这与雷帕霉素靶激酶在慢性低硫条件下控制植物代谢和细胞生长和分裂中的作用一致。此外，低硫降低了几个NLP转录因子的表达，这些转录因子是硝酸盐传感的主要参与者。最后，转录组和蛋白质组数据表明，低硫抑制芥子油苷的合成，低氮加剧芥子油苷的降解。这表明硫甙作为缓冲分子对氮和硫管理的重要性。
Plants have fundamental dependences on nitrogen and sulfur and frequently have to cope with chronic limitations when their supply is sub-optimal. This study aimed at characterizing the metabolomic, proteomic, and transcriptomic changes occurring in Arabidopsis leaves under chronic nitrate (Low-N) and chronic sulfate (Low-S) limitations in order to compare their effects, determine interconnections, and examine strategies of adaptation. Metabolite profiling globally revealed opposite effects of Low-S and Low-N on carbohydrate and amino acid accumulations, whilst proteomic data showed that both treatments resulted in increases in catabolic processes, stimulation of mitochondrial and cytosolic metabolism, and decreases in chloroplast metabolism. Lower abundances of ribosomal proteins and translation factors under Low-N and Low-S corresponded with growth limitation. At the transcript level, the major and specific effect of Low-N was the enhancement of expression of defence and immunity genes. The main effect of chronic Low-S was a decrease in transcripts of genes involved in cell division, DNA replication, and cytoskeleton, and an increase in the expression of autophagy genes. This was consistent with a role of target-of-rapamycin kinase in the control of plant metabolism and cell growth and division under chronic Low-S. In addition, Low-S decreased the expression of several NLP transcription factors, which are master actors in nitrate sensing. Finally, both the transcriptome and proteome data indicated that Low-S repressed glucosinolate synthesis, and that Low-N exacerbated glucosinolate degradation. This showed the importance of glucosinolate as buffering molecules for N and S management.