IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells.
IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells.
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DOI：
10.1186/1471-2407-14-480
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发表时间：
2014-07-03
期刊：
影响因子：
3.8
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Li S;Wang W;Zhang N;Ma T;Zhao C
Wnt5a的过度表达和巨噬细胞的浸润都与炎症和癌症有关。本研究旨在揭示Wnt5a在胃癌巨噬细胞募集中的作用。用实时荧光定量聚合酶链式反应检测胃癌组织和细胞中的mRNA表达。用酶联免疫吸附试验检测胃癌细胞的蛋白质分泌。用PcDNA3.1-Wnt5a表达载体和Wnt5a siRNA载体分别在胃细胞中过表达和沉默Wnt5a。用Transwell分析巨噬细胞的迁移情况，用FITC-鬼臼蛋白染色观察巨噬细胞的细胞骨架。WNT5A在胃癌组织中高表达，并分别与单核细胞趋化蛋白-1和白细胞介素1β(IL-1β)相关。在胃癌细胞中，WNT5a诱导单核细胞趋化蛋白-1的表达，这种表达是由IL-1β介导的。转染Wnt5a的胃癌细胞条件培养液通过单核细胞趋化蛋白-1(MCP-1)刺激巨噬细胞趋化和细胞骨架的改变，并被重组IL-1受体拮抗剂(rIL-1ra)抑制。提示Wnt5a通过上调MCP-1参与巨噬细胞募集，IL-1ra可能用于抑制胃癌的巨噬细胞募集。
Both Wnt5a overexpression and macrophage infiltration have been implicated in inflammation and cancer. The aim of this study is to reveal the involvement of Wnt5a in macrophage recruitment in gastric cancer. mRNA expression in gastric cancer tissues and cells was investigated by real-time PCR. Protein secretion by gastric cancer cells was determined by ELISA. PcDNA3.1-Wnt5a expression vector and Wnt5a siRNA vector were used to overexpress and silence Wnt5a expression in gastric cells, respectively. Macrophage migration was analyzed by transwell, and macrophage cytoskeleton was stained with FITC-phalloidin. Wnt5a was overexpressed in gastric cancer tissues, and correlated with monocyte chemotactic protein 1 (MCP-1) and interleukin 1β (IL-1β), respectively. In gastric cancer cells, Wnt5a induced MCP-1 expression, which was mediated by IL-1β. Conditioned medium from gastric cancer cells transfected with Wnt5a stimulated macrophage chemotaxis and cytoskeletal changes via MCP-1, which were suppressed by recombinant IL-1 receptor antagonist (rIL-1Ra). These results suggest that Wnt5a is involved in macrophage recruitment by upregulating MCP-1, and IL-1Ra may be used to inhibit macrophage recruitment in gastric cancer.
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影响因子：
3.8
作者：
Zhao C;Lu X;Bu X;Zhang N;Wang W
通讯作者：
Wang W
1,25-DiHydroxyvitamin D3 抑制细胞因子诱导的 MCP-1 分泌，并减少人类前脂肪细胞对单核细胞的募集。
影响因子：
4.9
作者：
Gao, D.;Trayhurn, P.;Bing, C.
通讯作者：
Bing, C.
影响因子：
4.4
作者：
Thorley, Andrew J.;Ford, Paul A.;Tetley, Teresa D.
通讯作者：
Tetley, Teresa D.
影响因子：
3.7
作者：
Nanbara H;Wara-aswapati N;Nagasawa T;Yoshida Y;Yashiro R;Bando Y;Kobayashi H;Khongcharoensuk J;Hormdee D;Pitiphat W;Boch JA;Izumi Y
通讯作者：
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影响因子：
11.2
作者：
Kurayoshi, Manabu;Oue, Naohide;Kikuchi, Akira
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