GroEL from the endosymbiont Buchnera aphidicola betrays the aphid by triggering plant defense
GroEL from the endosymbiont Buchnera aphidicola betrays the aphid by triggering plant defense
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DOI：
10.1073/pnas.1407687111
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发表时间：
2014-06-17
影响因子：
11.1
通讯作者：
Kaloshian, Isgouhi
中科院分区：
文献类型：
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作者：
Chaudhary, Ritu;Atamian, Hagop S.;Kaloshian, Isgouhi
蚜虫是一种以汁液为食的植物害虫，含有内共生体 Buchnera aphidicola，这对于它们的繁殖力和生存至关重要。在植物渗透和取食过程中，蚜虫会分泌唾液，其中含有预计会改变植物防御和新陈代谢的蛋白质。植物识别微生物相关的分子模式并诱导模式触发免疫（PTI）。尚未确定与蚜虫相关的分子模式。通过质谱分析，我们在马铃薯蚜虫 (Macrosiphum euphorbiae) 的唾液中鉴定出 105 种蛋白质，其中一些源自 Buchnera，包括伴侣蛋白 GroEL。由于 GroEL 是一种广泛保守的细菌蛋白，具有重要功能，因此我们测试了它在 PTI 中的作用。将 GroEL 应用或渗透到拟南芥 (Arabidopsis thaliana) 叶子上可诱导氧化爆发和 PTI 早期标记基因的表达。这些 GroEL 诱导的防御反应需要已知的辅助受体油菜素类固醇不敏感 1 相关受体激酶 1。此外，在转基因拟南芥植物中，groEL 的诱导表达激活了 PTI 标记基因的表达。此外，表达groEL的拟南芥植物表现出桃蚜（Myzus persicae）的繁殖力降低，表明对蚜虫的抵抗力增强。此外，通过基因工程表达 III 型分泌系统的荧光假单胞菌将 GroEL 递送至番茄（Solanum lycopersicum）或拟南芥中，也分别降低了马铃薯蚜虫和绿桃蚜虫的繁殖力。总的来说，我们的数据表明 GroEL 是一种触发 PTI 的分子模式。
Aphids are sap-feeding plant pests and harbor the endosymbiont Buchnera aphidicola, which is essential for their fecundity and survival. During plant penetration and feeding, aphids secrete saliva that contains proteins predicted to alter plant defenses and metabolism. Plants recognize microbe-associated molecular patterns and induce pattern-triggered immunity (PTI). No aphid-associated molecular pattern has yet been identified. By mass spectrometry, we identified in saliva from potato aphids (Macrosiphum euphorbiae) 105 proteins, some of which originated from Buchnera, including the chaperonin GroEL. Because GroEL is a widely conserved bacterial protein with an essential function, we tested its role in PTI. Applying or infiltrating GroEL onto Arabidopsis (Arabidopsis thaliana) leaves induced oxidative burst and expression of PTI early marker genes. These GroEL-induced defense responses required the known coreceptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1. In addition, in transgenic Arabidopsis plants, inducible expression of groEL activated PTI marker gene expression. Moreover, Arabidopsis plants expressing groEL displayed reduced fecundity of the green peach aphid (Myzus persicae), indicating enhanced resistance against aphids. Furthermore, delivery of GroEL into tomato (Solanum lycopersicum) or Arabidopsis through Pseudomonas fluorescens, engineered to express the type III secretion system, also reduced potato aphid and green peach aphid fecundity, respectively. Collectively our data indicate that GroEL is a molecular pattern that triggers PTI.