Relation Between the Viral Load Accumulation of Southern Rice Black-Streaked Dwarf Virus and the Different Developmental Stages of Sogatella furcifera (Hemiptera: Delphacidae)
Relation Between the Viral Load Accumulation of Southern Rice Black-Streaked Dwarf Virus and the Different Developmental Stages of Sogatella furcifera (Hemiptera: Delphacidae)
复制标题
南方水稻黑条矮缩病毒病毒载量积累与白背飞虱不同发育阶段的关系
DOI：
10.1093/jee/tov065
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发表时间：
2015-06-01
影响因子：
2.2
通讯作者：
Liu, Yu-Di
中科院分区：
文献类型：
--
作者：
An, Xing-Kui;Hou, Mao-Lin;Liu, Yu-Di
白背飞虱Sogatella furcifera（Horvath）是南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus，SRBSDV）的唯一传播媒介。本研究采用绝对定量qPCR方法检测了白背飞虱取食SRBSDV感染水稻植株后不同发育阶段病毒基因mRNA的表达水平。SRBSDV S10基因在同一发育阶段的病毒拷贝数的比较表明，白背飞虱有较高的病毒拷贝数时，获得的病毒在较早的发育阶段。在第一-第二若虫阶段获得病毒的成虫阶段白背飞虱比在第三-第四若虫阶段、第五若虫阶段和成虫阶段获得病毒的白背飞虱显示出显著更高的病毒滴度。第五若虫阶段的白背飞虱，获得病毒在第一，第二若虫阶段比第五若虫阶段的白背飞虱，获得病毒在第三和第四若虫阶段和第五若虫阶段的病毒拷贝数更高。病毒载量最高值出现在成虫中期。白背飞虱的年迁入特性有利于SRBSDV的扩散，因为该病毒可随强飞虱迁飞向远处传播。因此，调查SRBSDV阳性个体在不同生命阶段的病毒载量的变化，需要开发更有效的控制SRBSDV在该领域的传播。
The white-backed planthopper, Sogatella furcifera (Horvath), is currently the only confirmed vector of Southern rice black-streaked dwarf virus (SRBSDV), which causes severe rice production losses in China. In this study, an absolute quantification qPCR method was used to detect viral gene mRNA expression levels at different developmental stages of white-backed planthoppers fed SRBSDV-infected rice plants. A comparison of viral copy numbers of the SRBSDV S10 gene at the same developmental stage indicated that the white-backed planthopper had higher viral copy numbers when the virus was acquired at the earlier developmental stages. The adult-stage white-backed planthoppers that had acquired the virus at the first-second nymphal stage displayed significantly higher viral titers than white-backed planthoppers that acquired the virus at the third-fourth nymphal stage, at the fifth nymphal stage, and at the adult stage. The fifth nymphal stage white-backed planthoppers that acquired the virus at the first-second nymphal stage displayed higher viral copy numbers than fifth nymphal stage white-backed planthoppers that acquired the virus at the third-fourth nymphal stage and at the fifth nymphal stage. The highest viral load value appeared in the middle adult stage. The annual immigration characteristics of white-backed planthoppers would be beneficial for the dispersal of SRBSDV because this virus could be transmitted far away following the migration of vigorous planthoppers. Therefore, investigating the change in the viral load at different life stages of SRBSDV-positive individuals is required to develop more effective control of the spread of SRBSDV in the field.