D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma
D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma
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DOI：
10.1016/j.humpath.2005.01.019
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发表时间：
2005-04-01
期刊：
影响因子：
3.3
通讯作者：
Ordóñez, NG
中科院分区：
文献类型：
--
作者：
Ordóñez, NG
最近的研究表明，podoplanin和D2-40单克隆抗体，与胎儿生殖细胞中存在的癌胚抗原反应，是高度可靠的淋巴管内皮标志物。这两种标记物也在正常和反应性间皮瘤细胞中表达的观察结果促使人们研究它们在间皮瘤诊断中的潜在价值。为了确定podoplanin和D2-40是否在这些肿瘤的诊断中有任何用途，40例间皮瘤患者，（29例上皮样、5例双相和6例肉瘤样），34例肺癌（24例腺癌，10例鳞癌），80例非肺腺癌（17例卵巢、10例乳腺、10例结肠、10例肾脏、5例子宫内膜、5例胃、5例胰腺、5例前列腺、3例甲状腺）、12例滑膜肉瘤（6例双相和6例双相），5例血管瘤和2例腺瘤样肿瘤用podoplanin单克隆抗体和D2-40抗体免疫染色。D2-40和podoplanin的反应性在29例上皮样间皮瘤中的25例（86%）中获得，但在癌或肉瘤样间皮瘤中均未获得。D2-40和podoplanin阳性也见于4/5的双相间皮瘤和4/6的滑膜肉瘤的上皮样成分，而梭形细胞成分这些肿瘤是阴性的，作为单相滑膜肉瘤。5例血管肉瘤中有2例（40%）表达这些标记物，从而证实了以前的报道，即一些血管肉瘤可能具有淋巴管内皮分化。这两个腺瘤样肿瘤也是阳性的D2-40和podoplanin，这一发现提供了进一步的支持，这些肿瘤的间皮起源。得出的结论是，由于D2-40和podoplanin对上皮样间皮瘤具有高度的特异性和敏感性，因此它们是诊断这些肿瘤非常有用的标志物。与目前可用的其他标记相比，在我看来，它们似乎是最好的。(c)2005年爱思唯尔公司All rights reserved.
Recent investigations have shown that podoplanin and the D2-40 monoclonal antibody, which reacts with an oncofetal antigen present in fetal germ cells, are highly reliable lymphatic endothelial markers. The observation that both of these markers are also expressed in normal and reactive mesothelial cells prompted an investigation into their potential value in the diagnosis of mesotheliomas. To determine whether podoplanin and D2-40 had any use in the diagnosis of these tumors, 40 mesotheliomas (29 epithelioid, 5 biphasic, and 6 sarcomatoid), 34 carcinomas of the lung (24 adenocarcinomas, 10 squamous carcinomas), 80 nonpulmonary adenocarcinomas (17 ovary, 10 breast, 10 colon, 10 kidney, 5 endometrium, 5 stomach, 5 pancreas, 5 prostate, 3 thyroid), 12 synovial sarcomas (6 biphasic and 6 monophasic), 5 angiosarcomas, and 2 adenomatoid tumors were immunostained with a monoclonal antibody to podoplanin and with the D2-40 antibody. Reactivity for both D2-40 and podoplanin was obtained in 25 (86%) of the 29 epithelioid mesotheliomas but in none of the carcinomas or sarcomatoid mesotheliomas. Positivity for D2-40 and podoplanin was also seen in the epithelioid components of 4 of 5 biphasic mesotheliomas and 4 of 6 synovial sarcomas, whereas the spindle cell components of these tumors were negative as were the monophasic synovial sarcomas. Two (40%) of the 5 angiosarcomas expressed these markers, thus confirming previous reports suggesting that some angiosarcomas may have lymphatic endothelial differentiation. Both of the adenomatoid tumors were also positive for D2-40 and podoplanin, a finding which provides further support for the mesothelial derivation of these tumors. It is concluded that, because of their high specificity and sensitivity for epithelioid mesotheliomas, D2-40 and podoplanin are very useful markers for the diagnosis of these tumors. When compared with the other markers that are currently available, in my opinion, they appear to be the best. (c) 2005 Elsevier Inc. All rights reserved.