The effects of changes in the environment on the spatial firing of hippocampal complex-spike cells
The effects of changes in the environment on the spatial firing of hippocampal complex-spike cells
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DOI：
10.1523/jneurosci.07-07-01951.1987
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发表时间：
1987-07
期刊：
影响因子：
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通讯作者：
R. Muller;J. Kubie
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文献类型：
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作者：
R. Muller;J. Kubie
使用在前面的论文（Muller等人，1987年），我们研究了位置细胞对动物环境变化的反应。所用的标准装置是直径76厘米、壁高51厘米的圆筒。室内是统一的灰色，除了一个白色提示卡，运行的墙壁的整个高度，并占据了100度的弧。仪器的地板对动物的运动没有任何障碍。该装置的这些主要特征中的每一个都是变化的，而其他特征保持不变。其中一组操作涉及提示卡。旋转提示卡产生了单个细胞的发射场的相等旋转。改变卡片的宽度并不影响射野的大小、形状或径向位置，尽管有时射野会适度旋转。完全移除提示卡也使发射场的大小、形状和径向位置保持不变，但导致场旋转到不可预测的角度位置。第二组操作涉及器械壁的大小和形状。当标准（小）圆柱体的直径和高度按比例放大2倍时，在两个圆柱体中观察到的36%的细胞的发射场也按比例放大，在这个意义上，场保持在相同的角位置和相同的相对径向位置。在两个气缸中记录的细胞中，52%的细胞在一个气缸中表现出非常不同的放电模式。其余12%的细胞在两个圆筒中几乎是沉默的。当单个细胞在一个小的和一个大的矩形外壳中记录时，得到了类似的结果。相比之下，当装置平面布置图从圆形改变为矩形时，一种形状的装置中的单元的激发图案不能根据另一形状中的激发图案的知识来预测。最后的操作涉及将垂直障碍物放置到小圆柱体的无障碍地板上。当一个不透明的障碍物被设置成将先前记录的射击场一分为二时，几乎在所有情况下，射击场几乎都被取消了。这是真的，即使屏障只占射击场面积的一小部分。在衰减射野方面，透明屏障与不透明屏障一样有效。用于锚垂直屏障的电极导线底座不影响定位细胞放电。（400字处删节）
Using the techniques set out in the preceding paper (Muller et al., 1987), we investigated the response of place cells to changes in the animal's environment. The standard apparatus used was a cylinder, 76 cm in diameter, with walls 51 cm high. The interior was uniformly gray except for a white cue card that ran the full height of the wall and occupied 100 degrees of arc. The floor of the apparatus presented no obstacles to the animal's motions. Each of these major features of the apparatus was varied while the others were held constant. One set of manipulations involved the cue card. Rotating the cue card produced equal rotations of the firing fields of single cells. Changing the width of the card did not affect the size, shape, or radial position of firing fields, although sometimes the field rotated to a modest extent. Removing the cue card altogether also left the size, shape, and radial positions of firing fields unchanged, but caused fields to rotate to unpredictable angular positions. The second set of manipulations dealt with the size and shape of the apparatus wall. When the standard (small) cylinder was scaled up in diameter and height by a factor of 2, the firing fields of 36% of the cells observed in both cylinders also scaled, in the sense that the field stayed at the same angular position and at the same relative radial position. Of the cells recorded in both cylinders, 52% showed very different firing patterns in one cylinder than in the other. The remaining 12% of the cells were virtually silent in both cylinders. Similar results were obtained when individual cells were recorded in both a small and a large rectangular enclosure. By contrast, when the apparatus floor plan was changed from circular to rectangular, the firing pattern of a cell in an apparatus of one shape could not be predicted from a knowledge of the firing pattern in the other shape. The final manipulations involved placing vertical barriers into the otherwise unobstructed floor of the small cylinder. When an opaque barrier was set up to bisect a previously recorded firing field, in almost all cases the firing field was nearly abolished. This was true even though the barrier occupied only a small fraction of the firing field area. A transparent barrier was effective as the opaque barrier in attenuating firing fields. The lead base used to anchor the vertical barriers did not affect place cell firing.(ABSTRACT TRUNCATED AT 400 WORDS)