Effects of the tetrahedral framework nucleic acids on the skeletal muscle regenerationin vitroandin vivo
Effects of the tetrahedral framework nucleic acids on the skeletal muscle regenerationin vitroandin vivo
复制标题
四面体骨架核酸对体内外骨骼肌再生的影响
DOI：
10.1039/d0qm00329h
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发表时间：
2020-09-01
影响因子：
7
通讯作者：
Cai, XiaoXiao
中科院分区：
文献类型：
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作者：
Gao, Yang;Zhang, Tianxu;Cai, XiaoXiao
与肌肉退行性疾病和容积性肌肉丢失(VML)相关的挑战强调了肌肉组织再生的前景。尤其是为了尽快产生足够数量的细胞，维持成肌细胞的干性和自我更新是必不可少的，这就突出了成肌细胞数量和质量的重要性。然而，由于肌肉修复能力的下降，衰老细胞状态的变化逐渐显现。目前，在缺乏一种令人满意的方法来维持肌肉再生能力的情况下，发现促进肌肉再生中成肌细胞活性的药物仍然是实验研究的目标。四面体骨架核酸(TFNAs)可以调节细胞的增殖和迁移等多种生物学行为，在组织工程中具有巨大的潜力。在本研究中，我们研究了tFNAs对成肌细胞的增殖、自噬调节、干性维持、相关的潜在机制以及体内骨骼肌修复的影响。我们的结果表明，tFNAs通过Wnt/β-catenin信号通路促进成肌细胞增殖。此外，暴露于tFNAs后，成肌细胞的自噬水平被上调，茎的数量也保持不变。动物实验进一步证明，在急性肌肉损伤中，tFNAs可以促进骨骼肌再生。因此，基于tFNAs的应用为增强成肌细胞活性和促进骨骼肌再生提供了新的思路。
The challenges associated with muscle degenerative diseases and volumetric muscle loss (VML) emphasizes the prospects of muscle tissue regeneration. Especially, it is essential to maintain the stemness and self-renewal of myoblasts for generating an adequate number of cells as quickly as possible, which highlights the importance of the quantity and quality of myoblasts. However, changes in aged cellular status are gradually manifested due to decline in muscle repair capacity. Currently, in the absence of a satisfactory approach to maintain muscle regeneration capacity, the discovery of drugs for promoting myoblast activity in muscle regeneration remains the goal of experimental research. Tetrahedral framework nucleic acids (tFNAs) can regulate various biological behaviors of cells, including proliferation and migration, which imply a great potential in tissue engineering. In the present study, we investigated the effects of tFNAs on proliferation, autophagy regulation, stemness maintenance, and associated potential mechanism of myoblasts, and their skeletal muscle repairmentin vivo. Our results revealed that tFNAs promoted the proliferation of myoblastsviathe Wnt/beta-catenin signal pathway. Moreover, the autophagy level of myoblasts was upregulated and the stemness was also maintained upon exposure to tFNAs. Further, the animal experiments demonstrated that tFNAs could accelerate skeletal muscle regeneration in acute muscle injury. Therefore, a new idea for enhancing myoblast activity and promoting skeletal muscle regeneration based on the application of tFNAs is provided.