Characterization of cell binding and thrombin inhibitory regions on kininogens' heavy chain.
Characterization of cell binding and thrombin inhibitory regions on kininogens' heavy chain.
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激肽原重链上细胞结合和凝血酶抑制区域的表征。
DOI：
10.1007/978-3-0348-7321-5_31
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Schmaier,AH
中科院分区：
文献类型：
--
作者：
Jiang,YP;Schmaier,AH
已经进行了研究以确定激肽原重链上与血小板结合并含有凝血酶抑制区结构域3（但不含结构域1或2）的结构域完全抑制125 I-HK与血小板的结合（Ki= 24 nM+/-7，n= 4）。D3与血小板的结合是可饱和的，表观Kd为39 nM+/-8（n= 4），1227+/-404个结合位点/血小板。D3抑制125 I-凝血酶与血小板的结合，从而阻止凝血酶诱导的血小板聚集和分泌。这些研究表明，激肽原重链上的D3含有一个细胞结合区和另一个抑制凝血酶结合和血小板活化的区域。
Investigations have been performed to determine the domain (s) on kininogens' heavy chain that binds to platelets and contains thrombin inhibitory region Domain 3, but not domains 1 or 2, completely inhibited 125I-HK binding to platelets (Ki= 24 nM+/-7, n= 4). D3 binding to platelets was saturable with an apparent Kd of 39 nM+/-8 (n= 4) and 1227+/-404 binding sites/platelet. D3 inhibited 125I-thrombin binding to platelets which prevented thrombin-induced platelet aggregation and secretion. These studies indicate that D3 on kininogens' heavy chain contains a cell binding region and another region which inhibits thrombin binding and activation of platelets.