Effects of ribosomes on the kinetics of Qb replication
Effects of ribosomes on the kinetics of Qb replication
复制标题
核糖体对 Qb 复制动力学的影响
DOI：
10.1016/j.febslet.2013.11.018
复制
发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
T
中科院分区：
文献类型：
--
作者：
Usui;K.;Ichihashi;N.;Kazuta;Y.;Matsuura;T.;Yomo;T
噬菌体 Qβ 在复制过程中利用一些宿主细胞翻译因子。之前，我们构建了一个动力学模型来解释 Qβ 复制酶对长 RNA 分子的复制。在这里，我们扩展了之前的动力学模型，纳入了核糖体浓度对 RNA 复制的影响。扩展模型定量解释了两种人工长RNA在不同核糖体浓度复制过程中的单链和双链形成动力学。这一扩展模型和本研究中获得的知识为理解 Qβ 复制酶与核糖体的精确复制机制以及设计翻译偶联系统中的可扩增 RNA 基因组提供了有用的框架。
Bacteriophage Qβ utilizes some host cell translation factors during replication. Previously, we constructed a kinetic model that explains replication of long RNA molecules by Qβ replicase. Here, we expanded the previous kinetic model to include the effects of ribosome concentration on RNA replication. The expanded model quantitatively explained single- and double-strand formation kinetics during replication with various ribosome concentrations for two artificial long RNAs. This expanded model and the knowledge obtained in this study provide useful frameworks to understand the precise replication mechanism of Qβ replicase with ribosomes and to design amplifiable RNA genomes in translation-coupling systems.