Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase
Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase
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影响脱氧核糖核酸旋转酶的药物对基因表达的调节
DOI：
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发表时间：
1979
影响因子：
3.2
通讯作者：
Dipartement de Biochimie
中科院分区：
文献类型：
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作者：
Dipartement de Biochimie
萘二甲酸（Nal）是一种影响脱氧核糖核酸促旋酶活性的药物，可抑制代谢产物敏感基因的表达：三种麦芽糖操纵子、乳糖和半乳糖操纵子以及β-葡聚糖酶基因。已经观察到对Nal的敏感度与对分解代谢物阻遏的敏感度之间的相关性。苏氨酸和色氨酸操纵子的表达对分解代谢产物阻遏不敏感，对NaI不敏感。在IQ启动子控制下的lacZ基因的表达被NaI激活。在nalA基因座中携带突变的菌株对这些效应具有抗性。新生霉素抑制脱氧核糖核酸促旋酶的负超螺旋活性，影响操纵子的表达类似于Nal。参与的启动子在NAL和新生霉素的行动，以及在体内负超螺旋的基因表达的选择性的可能作用，进行了讨论。
Nalidixic acid (Nal), a drug which affects deoxyribonucleic acid gyrase activity, inhibits the expression of catabolite-sensitive genes: the three maltose operons, the lactose and galactose operons, and the tryptophanase gene. A correlation between the degree of sensitivity to Nal and that to catabolite repression has been observed. The expression of the threonine and tryptophan operons, insensitive to catabolite repression, is insensitive to Nal. The expression of the lacZ gene under the control of the IQ promoter is activated by Nal. Strains carrying a mutation in the nalA locus are resistant to these effects. Novobiocin, which inhibits the negative supercoiling activity of deoxyribonucleic acid gyrase, affects expression of the operons similarly to Nal. The involvement of promoters in Nal and novobiocin action, as well as a possible role of in vivo negative supercoiling in the selectivity of gene expression, are discussed.