Relationship between vascular development and vascular differentiation during liver organogenesis in humans
Relationship between vascular development and vascular differentiation during liver organogenesis in humans
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DOI：
10.1016/s0168-8278(02)00282-9
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发表时间：
2002-12-01
影响因子：
25.7
通讯作者：
Scoazec, JY
中科院分区：
文献类型：
--
作者：
Gouysse, G;Couvelard, A;Scoazec, JY
背景/目的：正常成人肝脏的复杂血管结构特征在胎儿期逐渐获得。在这项研究中，我们的目的是评估血管生成和血管分化之间的关系，在肝脏器官genes.Methods：我们研究了，在51个不同胎龄的胎儿，内皮细胞分化的标志物，整合素，促血管生成和抗血管生成的细胞外基质成分，血管内皮生长因子（VEGF）及其受体的表达。肝脏血管结构的发育主要分为三个阶段：（a）妊娠5 ~ 10周（GW），未检测到新生血管形成的证据;肝原基中的血管是门静脉和血窦的前体，由先存血管衍生而来;（B）从10到25 GW，血管生成和血管发生分别导致动脉和门静脉内毛细血管的发育，而门静脉和肝窦则遵循分化过程;（c）25 GW后，在各种血管隔室中检测到很少的变化。VEGF及其受体的最大表达量为5 ~ 25 GW。结论：肝血管构筑的发育是一个由血管生成、血管生成和血管分化组成的多步骤过程，受VEGF等特异性生长和分化因子的调控。(C)2002年欧洲肝脏研究协会。由Elsevier Science B. V.出版，版权所有。
Backgrounds/Aims: The complex vascular architecture characteristic of the normal adult liver is progressively acquired during the fetal life. In this study, we aimed to evaluate the relationship between angiogenesis and vascular differentiation during liver organogenesis.Methods: We studied, in 51 fetuses of different gestational ages, the expression of markers of endothelial cell differentiation, integrins, pro- and anti-angiogenic extracellular matrix components, vascular endothelial growth factor (VEGF) and its receptors.Results: Three main stages in the development of the vascular architecture of the liver were identified: (a) from 5 to 10 gestation weeks (GW), no evidence of de novo angiogenesis was detected; the vessels present in the liver primordium were the precursors of portal veins and sinusoids, deriving from preexisting vessels; (b) from 10 to 25 GW, angiogenesis and vasculogenesis resulted in the development of, respectively, arteries and intra-portal capillaries, while portal veins and hepatic sinusoids followed a differentiation process; (c) after 25 GW, little changes were detected in the various vascular compartments. The maximal expression of VEGF and its receptors was from 5 to 25 GW.Conclusions: The development of the hepatic vascular architecture is a multistep process combining angiogenesis, vasculogenesis and vascular differentiation, regulated by specific growth and differentiation factors including VEGF. (C) 2002 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.