Visualization of Hidden Epitopes at the Inactive X Chromosome.

Visualization of Hidden Epitopes at the Inactive X Chromosome.
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无活性 X 染色体上隐藏表位的可视化。

DOI:
10.1007/978-1-4939-8766-5_9
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发表时间:
2018
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Stewart ER
Stewart ER
中科院分区:
--
文献类型:
--
作者:
Stewart ER

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Immunodetection of nuclear antigens is often complicated by epitope masking, so that proteins known to function in the nucleus are sometimes not easily detected at their sites of action. Moreover, protein populations that are detected before unmasking can be very different to those seen after removal of nucleic acids. This is particularly true for components of the nuclear matrix, including those known to function at the inactive X chromosome. Here we describe an unmasking protocol that reveals previously undetected proteins at the inactive X chromosome in mouse fibroblasts.
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