A complement of ten essential and pleiotropic arabidopsis COP/DET/FUS genes is necessary for repression of photomorphogenesis in darkness
A complement of ten essential and pleiotropic arabidopsis COP/DET/FUS genes is necessary for repression of photomorphogenesis in darkness
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DOI：
10.1104/pp.110.3.731
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发表时间：
1996-03-01
期刊：
影响因子：
7.4
通讯作者：
Deng, XW
中科院分区：
文献类型：
--
作者：
Kwok, SF;Piekos, B;Deng, XW
两个遗传筛选，一个突变导致在黑暗中的光形态发育和其他突变体与fusca表型，迄今已确定6个多效性拟南芥COP/DET/FUS基因。在这里，我们的特点是代表性的突变体，定义四个额外的多效性光形态发生基因座和一个空突变等位基因的先前定义的DET 1基因座。这些隐性突变纯合的黑暗生长的幼苗表现出短的下胚轴和扩大的子叶，并在达到生殖发育之前是致命的。黑暗生长的突变体幼苗还显示特征性的光形态发生细胞分化和光诱导基因的表达升高。此外，从黑暗中生长的突变体的质体分析揭示部分叶绿体分化和不存在的叶绿体发育。光生长的突变体幼苗的根维管束细胞发育叶绿体，这表明这些FUS基因产物是重要的抑制光生长的根中的叶绿体分化。双突变体分析表明，这些多效性的COP/DET/FUS突变是上位突变的光敏色素，蓝光感光器，和下游的调节组件，HY 5。因此，至少有10个必需的和多效性的拟南芥基因是必需的光形态发生发育的抑制的补充。
Two genetic screens, one for mutations resulting in photomorphogenic development in darkness and the other for mutants with fusca phenotype, have thus far identified six pleiotropic Arabidopsis COP/DET/FUS genes. Here, we characterized representative mutants that define four additional pleiotropic photomorphogenic loci and a null mutant allele of the previously defined DET1 locus. Dark-grown seedlings homozygous for these recessive mutations exhibit short hypocotyls and expanded cotyledons and are lethal before reaching reproductive development. Dark-grown mutant seedlings also display characteristic photomorphogenic cellular differentiation and elevated expression of light-inducible genes. In addition, analyses of plastids from dark-grown mutants reveal partial chloroplast differentiation and absence of etioplast development. Root vascular bundle cells of light-grown mutant seedlings develop chloroplasts, suggesting that these FUS gene products are important for suppression of chloroplast differentiation in light-grown roots. Double-mutant analyses indicate that these pleiotropic cop/det/fus mutations are epistatic to mutations in phytochromes, a blue-light photoreceptor, and a downstream regulatory component, HY5. Therefore, there is a complement of at least 10 essential and pleiotropic Arabidopsis genes that are necessary for repression of photomorphogenic development.