DAF-1, A C-ELEGANS GENE CONTROLLING DAUER LARVA DEVELOPMENT, ENCODES A NOVEL RECEPTOR PROTEIN-KINASE
DAF-1, A C-ELEGANS GENE CONTROLLING DAUER LARVA DEVELOPMENT, ENCODES A NOVEL RECEPTOR PROTEIN-KINASE
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DOI：
10.1016/0092-8674(90)90475-t
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发表时间：
1990-05-18
期刊：
影响因子：
64.5
通讯作者：
RIDDLE, DL
中科院分区：
文献类型：
--
作者：
GEORGI, LL;ALBERT, PS;RIDDLE, DL
Dauer幼虫是一个发育停滞，非喂养传播阶段，通常形成的过度拥挤和有限的食物。daf-1基因是一系列基因的中间步骤，这些基因被认为是环境信号的神经传导途径。daf-1突变导致即使在丰富的食物中也能组成性形成dauer幼虫。该基因已被克隆的Tc 1转座子标签，它似乎编码一类新的丝氨酸/苏氨酸激酶。daf-1探针检测到低丰度的2.5 kb mRNA，DNA序列表明该基因编码669个氨基酸的蛋白质，具有推定的跨膜结构域和与胞质raf原癌基因家族最密切相关的C-末端蛋白激酶结构域。因此，daf-1产物似乎是将环境信号转导成适当的发育反应所需的细胞表面受体。
The dauer larva is a developmentally arrested, non-feeding dispersal stage normally formed in response to overcrowding and limited food. The daf-1 gene specifies an intermediate step in a hierarchy of genes thought to specify a pathway for neural transduction of environmental cues. Mutations in daf-1 result in constitutive formation of dauer larvae even in abundant food. This gene has been cloned by Tc1-transposon tagging, and it appears to encode a new class of serine/threonine kinase. A daf-1 probe detects a 2.5 kb mRNA of low abundance, and the DNA sequence indicates that the gene encodes a 669 amino acid protein, with a putative transmembrane domain and a C-terminal protein kinase domain most closely related to the cytosolic, raf proto-oncogene family. Hence, the daf-1 product appears to be a cell-surface receptor required for transduction of environmental signals into an appropriate developmental response.