ROLE OF ARGININE DEIMINASE IN GROWTH OF MYCOPLASMA-HOMINIS
ROLE OF ARGININE DEIMINASE IN GROWTH OF MYCOPLASMA-HOMINIS
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DOI：
10.1128/jb.126.1.501-510.1976
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发表时间：
1976-01-01
影响因子：
3.2
通讯作者：
KENNY, GE
中科院分区：
文献类型：
--
作者：
FENSKE, JD;KENNY, GE
精氨酸被认为是非糖酵解精氨酸利用支原体的主要能源。当3株精氨酸分枝杆菌和1株节炎分枝杆菌、发酵分枝杆菌、鸡分枝杆菌、鸡分枝杆菌和人分枝杆菌分别在高精氨酸浓度(34 MM)和低精氨酸浓度(4 MM)的培养基中生长时，微生物的蛋白质含量和精氨酸脱亚胺酶的比活力都增加，而1种同时也是糖酵解的精氨酸利用种发酵分枝杆菌的蛋白质含量增加，但酶的比活力没有增加。糖酵解、非精氨酸利用的鸡败血分枝杆菌在这两个参数中都没有表现出增加。从细胞粗提物中提取的精氨酸脱亚胺酶的Km为1.66倍。10~(-4)M，底物抑制时，该酶呈双曲线激活曲线，不受L组氨酸的影响。当在好氧和厌氧条件下测定人型支原体的支原体蛋白和精氨酸脱亚胺酶时，好氧培养的细胞直到对数生长期后期才表现出可检测到的酶活性增加。在厌氧生长周期的早期观察到较高水平的精氨酸脱亚胺酶。与低精氨酸培养的细胞相比，添加精氨酸的细胞从[Gu-14C]精氨酸放出14CO2的速率没有改变。CO2的产生与精氨酸脱亚胺酶活性的升高并不平行。在这些生物体中，精氨酸显然只被用作替代能源。
Arginine was considered as the major energy source of nonglycolytic arginine-utilizing mycoplasmata. When 3 strains of M. arginini, and 1 strain each of M. arthritidis, M. fermentans, M. gallinarum, M. gallisepticum and M. hominis were grown in the medium with high arginine concentration (34 mM) compared with low arginine (4 mM), both the protein content of the organisms and the specific activity of arginine deiminase increased, M. fermentans, the 1 arginine-utilizing species included in the survey which is also glycolytic, showed an increase in protein content but no increase in specific activity of the enzyme. The glycolytic non-arginine-utilizing M. gallisepticum did not show an increase in either parameter. The Km for arginine deiminase from crude cell extracts was 1.66 .times. 10-4 M. The enzyme demonstrated a hyperbolic activation curve subject to substrate inhibition and was not affected by the presence of L-histidine. When mycoplasmic protein and arginine deiminase were determined for M. hominis under aerobic and anaerobic conditions, aerobically grown cells exhibited no detectable enzymatic increases until late in log phase. Higher levels of arginine deiminase were observed earlier in the anaerobic growth cycle. The rate of 14CO2 evolution from [guanido-14C]arginine was not altered in arginine-supplemented cells compared with cells grown in low arginine. CO2 production did not parallel increased arginine deiminase activity. Arginine is apparently used only as an alternate energy source in these organisms.