Organization and efferent projections of nucleus tegmenti pedunculopontinus pars compacta with special reference to its cholinergic aspects
Organization and efferent projections of nucleus tegmenti pedunculopontinus pars compacta with special reference to its cholinergic aspects
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被盖脚桥核致密部的组织和传出投射，特别涉及其胆碱能方面
DOI：
10.1016/0306-4522(84)90204-5
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
T. Hattori
中科院分区：
文献类型：
--
作者：
T. Sugimoto;T. Hattori
在试图评估的细胞组织和传出投射的被盖脚桥核部，几个实验进行了大鼠。从Nissl染色切片中的被盖脚桥核部神经元的测量中，观察到该核包含许多大神经元，这使得有可能将该核与周围的桥中脑网状结构区分开来。另外两个神经元群体，中型和小型神经元，也被认为是在被盖脚桥核部。详细的测量结果表明，90%的体积的所有神经元在被盖脚桥核部的大，中型神经元。将[~ 3 H]亮氨酸注入被盖脚桥核后，在背侧和腹侧上行纤维中观察到转运标记。背侧走行纤维进入丘脑中央外侧核和中央束旁核复合体。腹侧走行的纤维在腹侧被盖区、黑质背侧部、丘脑底核、齿状回和下丘脑外侧部产生银颗粒的聚集。在丘脑和后连合的水平上，观察到被盖脚桥核的交叉纤维稀疏，并在更大程度上通过Meynert的视上连合。在对侧大脑的等同终末部位，顺行标记少得多。通过电子显微镜放射自显影主要终端站点的神经轴突的被盖脚桥核部的检查大鼠注射[3 H]亮氨酸在被盖脚桥核部的背侧部，后来注射辣根过氧化物酶在纹状体和苍白球。统计数据显示，优先放射性标记的终端形成不对称的突触接触与树突的中央外侧核，中央束旁复合体和丘脑底核。在早期研究中提出的黑质视神经部的明显终止，并在目前的光镜放射自显影图中显示，不支持这种超微结构分析。几个放射性标记的终端的不对称型接触辣根过氧化物酶标记的树突在丘脑证实直接输入从被盖脚桥核部丘脑纹状体投射神经元。[3 H]胆碱注射到丘脑和丘脑底核产生逆行标记的大神经元在被盖脚桥核部的背侧核。在黑质注射[3 H]胆碱后，这些神经元未被标记。其他研究结果表明，[3 H]胆碱通过胆碱能末梢以及通道的胆碱能纤维的逆行运输。这些数据表明，选择性摄取机制的胆碱能纤维的passage.The结果强调了丘脑和丘脑底核的神经支配的被盖脚桥核的胆碱能性质。大的神经元在被盖脚桥核pars后叶似乎负责这种胆碱能神经支配，并可能提供轴突终末，使不对称的突触在丘脑和丘脑底核如上所述。此外，大的神经元以及中型和小型的被盖脚桥核pars rectata其发射器和确切的目的地仍然未知发送一些轴突通过…
In an attempt to evaluate the cellular organization and efferent projections of the nucleus tegmenti pedunculopontinus pars compacta, several experiments were performed in the rat. From measurements of neurons in the nucleus tegmenti pedunculopontinus pars compacta in Nissl-stained sections, the nucleus was observed to contain many large neurons which made it possible to demarcate this nucleus from surrounding pontomesencephalic reticular formation. Two other neuronal populations, medium and small neurons, were also seen in the nucleus tegmenti pedunculopontinus pars compacta. Detailed measurements showed that 90% by volume of all neurons in the nucleus tegmenti pedunculopontinus pars compacta were large and medium-sized neurons. After injections of [3H]leucine into the nucleus tegmenti pedunculopontinus pars compacta, transported label was observed in dorsally and ventrally coursing ascending fibers. The dorsally coursing fibers entered the centrolateral nucleus and centre median-parafascicular complex of the thalamus. The ventrally coursing fibers produced accumulation of silver grains in the ventral tegmental area, substantia nigra pars compacta, subthalamic nucleus, zona incerta and lateral hypothalamus. Crossed fibers of the nucleus tegmenti pedunculopontinus pars compacta were observed sparsely at the levels of the thalamus and posterior commissure, and to a greater degree through the supraoptic commissure of Meynert. Much less anterograde labeling was seen in the equivalent terminal sites on the contralateral side of the brain. By electron microscopic autoradiography major terminal sites of axons of the nucleus tegmenti pedunculopontinus pars compacta were examined in rats injected with [3H]leucine in the nucleus tegmenti pedunculopontinus pars compacta and later injected with horseradish peroxidase in the striatum and pallidum. Statistical data showed preferential radiolabeling of terminals forming asymmetrical synaptic contact with dendrites in the centrolateral nucleus, centre median-parafascicular complex and subthalamic nucleus. Apparent terminations in the substantia nigra pars compacta proposed in earlier studies and shown in the present light microscopic autoradiograms were not supported by this ultrastructural analysis. Several radiolabeled terminals of the asymmetrical type contacting horseradish peroxidase labeled dendrites in the thalamus confirmed direct input from the nucleus tegmenti pedunculopontinus pars compacta to the thalamostriate projection neurons. [3H]choline injections into the thalamus and subthalamic nucleus produced retrograde perikaryal labeling of large neurons in the nucleus tegmenti pedunculopontinus pars compacta. These neurons were unlabeled after [3H]choline injections in the substantia nigra. Other findings suggested retrograde transport of [3H]choline through cholinergic terminals as well as cholinergic fibers of passage. These data suggested a selective uptake mechanism for cholinergic fibers of passage.The results emphasize the cholinergic nature of the nucleus tegmenti pedunculopontinus pars compacta innervation of the thalamus and subthalamic nucleus. Large neurons in the nucleus tegmenti pedunculopontinus pars compacta seem responsible for this cholinergic innervation and probably provide the axon terminals making asymmetrical synapses in the thalamus and subthalamic nucleus as described above. In addition, large neurons as well as medium and small ones in the nucleus tegmenti pedunculopontinus pars compacta whose transmitters and exact destinations remain unknown send a number of axons through the …