Human SWI/SNF interconverts a nucleosome between its base state and a stable remodeled state
Human SWI/SNF interconverts a nucleosome between its base state and a stable remodeled state
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DOI：
10.1016/s0092-8674(00)81217-9
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发表时间：
1998-07-10
期刊：
影响因子：
64.5
通讯作者：
Kingston, RE
中科院分区：
文献类型：
--
作者：
Schnitzler, G;Sif, S;Kingston, RE
人SWI/SNF复合物以ATP依赖的方式重塑核小体结构，尽管这种变化的性质尚未确定。在这里，我们表明，hSWI/SNF和ATP产生一个改变的核小体结构是稳定的SWI/SNF的情况下。该产品对DNA酶、限制性内切酶和微球菌核酸酶消化的敏感性改变，对GAL 4的亲和力增加。它具有相同的蛋白质组成，但大小约为正常核小体的两倍。改变的核小体与hSWI/SNF的孵育在ATP依赖性过程中将该结构转化回标准核小体。这些结果表明，hSWI/SNF的作用，促进正常和改变，更容易，核小体构象之间的交换。
The human SWI/SNF complex remodels nucleosome structure in an ATP-dependent manner, although the nature of this change has not been determined. Here we show that hSWI/SNF and ATP generate an altered nucleosomal structure that is stable in the absence of SWI/SNF. This product has an altered sensitivity to digestion by DNAse, restriction enzymes, and micrococcal nuclease, and an increased affinity for GAL4. It has the same protein composition but is approximately twice the size of a normal nucleosome. Incubation of the altered nucleosome with hSWI/SNF converts this structure back to a standard nucleosome in an ATP-dependent process. These results suggest that hSWI/SNF acts by facilitating an exchange between normal and altered, more accessible, nucleosome conformations.