OMEGA-CONOTOXIN GVIA BLOCKS SYNAPTIC TRANSMISSION IN THE CA1 FIELD OF THE HIPPOCAMPUS
OMEGA-CONOTOXIN GVIA BLOCKS SYNAPTIC TRANSMISSION IN THE CA1 FIELD OF THE HIPPOCAMPUS
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DOI：
10.1016/0014-2999(89)90318-x
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发表时间：
1989-12-19
影响因子：
5
通讯作者：
LAMOUR, Y
中科院分区：
文献类型：
--
作者：
DUTAR, P;RASCOL, O;LAMOUR, Y
omega的作用。- concontoxin GVIA-CgTx)是一种从海洋软体动物的毒液中分离出来的肽，在大鼠海马神经元中进行了研究。Ca1区域的细胞内记录用于体外切片制备。-CgTx (0.1-1 .mu；M)迅速且不可逆地阻断了电刺激Schaffer侧枝/连接纤维引起的EPSP和ipsp。-CgTx还能阻断电刺激胆碱能传入引起的慢胆碱能EPSP。巴氯芬或卡巴醇的突触后效应在omega存在时保持不变。-CgTx和其他突触后钙依赖事件，如后超极化不受.omega.-CgTx的影响。这些结果表明- omega在突触前有作用。-CgTx通过阻断神经递质释放。-CgTx可能通过阻断突触前n型电压敏感钙通道在海马中起作用。
The effects of .omega.-conotoxin GVIA (.omega.-CgTx), a peptide isolated from the venom of a marine mollusc, were studied in rat hippocampal neurons. Intracellular recordings from the Ca1 area were made for the purpose in in vitro slice preparations. .omega.-CgTx (0.1-1 .mu.M) rapidly and irreversibly blocked the EPSP and the IPSPs elicited by electrical stimulation of Schaffer collaterals/commissural fibers. .omega.-CgTx also blocked the slow cholinergic EPSP induced by electrical stimulation of cholinergic afferents. The postsynaptic effects of baclofen or carbachol remained unchanged in the presence of .omega.-CgTx and other postsynaptic calcium-dependent events such as afterhyperpolarization were not affected by .omega.-CgTx. These results suggest a presynaptic action of .omega.-CgTx through the blockade of neurotransmitter release. .omega.-CgTx might act in the hippocampus by blocking presynaptic N-type voltage-sensitive calcium channels.