Roles of charged particles and reactive species on cell membrane permeabilization induced by atmospheric-pressure plasma irradiation
Roles of charged particles and reactive species on cell membrane permeabilization induced by atmospheric-pressure plasma irradiation
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DOI：
10.7567/jjap.55.07lg04
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发表时间：
2016-06
影响因子：
1.5
通讯作者：
S. Sasaki;M. Kanzaki;Yutaro Hokari;Kanako Tominami;T. Mokudai;H. Kanetaka;T. Kaneko
中科院分区：
文献类型：
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作者：
S. Sasaki;M. Kanzaki;Yutaro Hokari;Kanako Tominami;T. Mokudai;H. Kanetaka;T. Kaneko
作为直接和间接大气压等离子体照射过程中影响细胞膜透化的因素，评价了等离子体照射溶液中的带电粒子流入、超氧阴离子自由基（O2− H2）和过氧化氢（H2O2）。这是三个强有力的候选因素，并可能成倍有助于细胞膜透化。特别是，较短的等离子体扩散距离导致增强的直接效应，如带电粒子流入，并进一步增加细胞膜的通透性。此外，O2− H2在等离子体照射的水中随时间（几分钟的寿命）消散，并且在等离子体照射的溶液的细胞膜透化方面的失活在相同的寿命内发生。这些结果有助于进一步了解等离子体诱导细胞膜透化的机制。
As factors that influence cell membrane permeabilization during direct and indirect atmospheric-pressure plasma irradiation, charged particle influx, superoxide anion radicals (O2−∙), and hydrogen peroxide (H2O2) in plasma-irradiated solution were evaluated. These are the three strong candidate factors and might multiply contribute to cell membrane permeabilization. In particular, a shorter plasma diffusion distance leads to the enhancement of the direct effects such as charged particle influx and further increase cell membrane permeability. In addition, O2−∙ dissipates over time (a life span of the order of minutes) in plasma-irradiated water, and the deactivation of a plasma-irradiated solution in term of cell membrane permeabilization occurs in a life span of the same order. These results could promote the understanding of the mechanism of plasma-induced cell membrane permeabilization.