Comparison of the transcriptional landscapes between human and mouse tissues
Comparison of the transcriptional landscapes between human and mouse tissues
复制标题
DOI：
10.1073/pnas.1413624111
复制
发表时间：
2014-12-02
影响因子：
11.1
通讯作者：
Snyder, Michael P.
中科院分区：
文献类型：
--
作者：
Lin, Shin;Lin, Yiing;Snyder, Michael P.
尽管人类和小鼠之间在形态和遗传上的相似性通常得到强调，但仍存在许多差异。为了在分子水平上更好地了解这两个物种，我们通过深度 RNA 测序对 15 个组织的表达谱进行了比较，并检查了蛋白质编码和非编码转录本的转录组的相似性和差异。尽管两个物种之间的组织特异性基因表达存在明显的共性，但发现许多组基因的表达在同一物种内的不同组织中比物种之间更相似。相关的表观遗传组蛋白标记分析进一步证实了这些发现。我们还发现许多非编码转录本的表达水平较低，并且在个体中无法以明显的水平检测到。此外，大多数物种之间缺乏明显的序列同源性，即使我们将注意力限制在那些在生物复制中具有最高重现性的序列上。总的来说，我们的结果表明人类和小鼠之间存在相当大的 RNA 表达多样性，远远超出了之前的描述，这可能反映了这两种生物之间的基本生理差异。
Although the similarities between humans and mice are typically highlighted, morphologically and genetically, there are many differences. To better understand these two species on a molecular level, we performed a comparison of the expression profiles of 15 tissues by deep RNA sequencing and examined the similarities and differences in the transcriptome for both protein-coding and -noncoding transcripts. Although commonalities are evident in the expression of tissue-specific genes between the two species, the expression for many sets of genes was found to be more similar in different tissues within the same species than between species. These findings were further corroborated by associated epigenetic histone mark analyses. We also find that many noncoding transcripts are expressed at a low level and are not detectable at appreciable levels across individuals. Moreover, the majority lack obvious sequence homologs between species, even when we restrict our attention to those which aremost highly reproducible across biological replicates. Overall, our results indicate that there is considerable RNA expression diversity between humans and mice, well beyond what was described previously, likely reflecting the fundamental physiological differences between these two organisms.