Ultrasonic spectral parameter characterization of apoptosis
Ultrasonic spectral parameter characterization of apoptosis
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DOI：
10.1016/s0301-5629(02)00492-1
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发表时间：
2002-05-01
影响因子：
2.9
通讯作者：
Sherar, MD
中科院分区：
文献类型：
--
作者：
Kolios, MC;Czarnota, GJ;Sherar, MD
超声（US）光谱分析方法用于分析从暴露于诱导细胞凋亡的化疗药物和其他诱导核转化的化学物质的细胞颗粒中收集的射频（RF）数据。利用线性回归技术对感兴趣区域（ROI）校准后向散射光谱进行分析，计算光谱斜率和中带拟合。两个f/2换能器，工作频率为30和34 MHz（相对带宽分别为93%和78%），与定制的成像系统一起使用，可以收集原始RF数据。对于凋亡细胞，光谱斜率从药物暴露前的0.37 dB/MHz增加到24 h后的0.57 dB/MHz，对应于有效散射半径从8.7 ma增加到3.2 ma。中带配合随时间增加，暴露后24小时达到13dB峰值。光谱参数的统计偏差与理论预测非常吻合。该结果为使用光谱参数方法监测体外和体内系统的细胞凋亡提供了框架，并用于指导系统和信号分析参数的设计。(C) 2002年世界超声医学生物学联合会。
Ultrasound (US) spectral analysis methods are used to analyze the radiofrequency (RF) data collected from cell pellets exposed to chemotherapeutics that induce apoptosis and other chemicals that induce nuclear transformations. Calibrated backscatter spectra from regions-of-interest (ROI) were analyzed using linear regression techniques to calculate the spectral slope and midband fit. Two f/2 transducers, with operating frequencies of 30 and 34 MHz (relative bandwidths of 93% and 78%, respectively) were used with a custom-made imaging system that enabled the collection of the raw RF data. For apoptotic cells, the spectral slope increased from 0.37 dB/MHz before drug exposure to 0.57 dB/MHz 24 h after, corresponding to a change in effective scatterer radius from 8.7 to 3.2 mum. The midband fit increased in a time-dependent fashion, peaking at 13dB 24 h after exposure. The statistical deviation of the spectral parameters was in close agreement with theoretical predictions. The results provide a framework for using spectral parameter methods to monitor apoptosis in in vitro and in in vivo systems and are being used to guide the design of system and signal analysis parameters. (C) 2002 World Federation for Ultrasound in Medicine Biology.