LSD1 knockdown reveals novel histone lysine methylation in human breast cancer MCF-7 cells
LSD1 knockdown reveals novel histone lysine methylation in human breast cancer MCF-7 cells
复制标题
LSD1 敲除揭示了人乳腺癌 MCF-7 细胞中新的组蛋白赖氨酸甲基化
DOI：
10.1016/j.biopha.2017.05.106
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发表时间：
2017-08-01
影响因子：
7.5
通讯作者：
Hu, Xin
中科院分区：
文献类型：
--
作者：
Jin, Yue;Huo, Bo;Hu, Xin
Histone lysine methylation, which plays an important role in the regulation of gene expression, genome stability, chromosome conformation and cell differentiation, is a dynamic process that is collaboratively regulated by lysine methyltransferases (KMTs) and lysine demethylases (KDMs). LSD1, the first identified KDMs, catalyzes the demethylation of mono-and di-methylated H3K4 and H3K9. Here, we systematically investigated the effects of LSD1 knockdown on histone methylations. Surprisingly, in addition to H3K4 and H3K9, the methylation level on other histone lysines, such as H3K27, H3K36 and H3K79, are also increased. The expression of SOX2, E-cadherin and FoxA2 are increased upon LSD1 knockdown, and the methylation level of H3K4, H3K27 and H3K36 in the promoter region of these genes are all changed after LSD1 knockdown. Our results show that LSD1 knockdown has a broad effect on histone lysine methylation, which indicates that LSD1 regulates histone lysine methylation in collaboration with other KMTs and KDMs. (C) 2017 Elsevier Masson SAS. All rights reserved.
