Smart hydrogels containing adenylate kinase: translating substrate recognition into macroscopic motion.
Smart hydrogels containing adenylate kinase: translating substrate recognition into macroscopic motion.
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DOI：
10.1021/ja805634x
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发表时间：
2008-11-26
影响因子：
15
通讯作者：
Kopecek J
中科院分区：
文献类型：
--
作者：
Yuan W;Yang J;Kopecková P;Kopecek J
通过将含有两个硫醇基团的腺苷酸激酶突变体共价掺入HPMA共聚物或聚乙二醇基的水凝胶结构中，制备了基于酶的杂化水凝胶。由底物识别引发的酶的纳米级构象变化转化为水凝胶的宏观运动。
Enzyme-based hybrid hydrogels were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, into HPMA copolymer- or PEG-based hydrogel structures. The nanoscale conformational change of enzyme, triggered by substrate recognition, translated into macroscopicmotionof hydrogels.