A 300 MHz and 600 MHz proton NMR study of a 12 base pair restriction fragment: investigation of structure by relaxation measurements.
A 300 MHz and 600 MHz proton NMR study of a 12 base pair restriction fragment: investigation of structure by relaxation measurements.
复制标题
12 碱基对限制性片段的 300 MHz 和 600 MHz 质子 NMR 研究：通过弛豫测量研究结构。
DOI：
10.1093/nar/8.23.5795
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发表时间：
1980
影响因子：
14.9
通讯作者：
Wells,RD
中科院分区：
文献类型：
--
作者：
Early,TA;Kearns,DR;Hillen,W;Wells,RD
在300 MHz和600 MHz下测量了一个12碱基对DNA限制性片段的~1H核磁共振谱，并分别分辨了70多个质子的共振谱。在300 MHz上进行了驰豫速率的测量，并与各向同性刚性转子模型的理论预测进行了比较，该模型的坐标来源于DNA的Dreding模型。对于大多数质子，当旋转关联时间为7nsec时，该模型给出的结果与实验符合得很好，尽管对于某些碱性质子，通过对糖基使用较短的关联时间或通过增加糖基质子间的距离来改善一致性。对碳结合质子的非选择性和选择性自旋晶格驰豫速率的比较表明，即使在这个短的DNA片段中，也存在广泛的自旋扩散。对同一类型质子在不同碱基对上的自旋-自旋弛豫速率的研究表明，序列对构象的影响很小。
The1H NMR spectrum of a 12 base pair DNA restriction fragment has been measured at 300 and 600 MHz and resonances from over 70 protons are individually resolved. Relaxation rate measurements have been carried out at 300 MHz and compared with the theoretical predictions obtained using an isotropic rigid rotor model with coordinates derived from a Dreiding model of DNA. The model gives results that are in excellent agreement with experiment for most protons when a 7 nsec rotational correlation time is used, although agreement is improved for certain base protons by using a shorter correlation time for the sugar group, or by increasing the sugar-base interproton distances. A comparison of non-selective and selective spin-lattice relaxation rates for carbon bound protons indicates that there is extensive spin diffusion even in this short DNA fragment. Examination of the spin-spin relaxation rates for the same type of proton on different base pairs reveals little sequence effect on conformation.