Comparative analysis of the expression of ERBIN and Erb-B2 in normal human skin and cutaneous carcinomas
Comparative analysis of the expression of ERBIN and Erb-B2 in normal human skin and cutaneous carcinomas
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DOI：
10.1111/j.1365-2133.2005.06687.x
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发表时间：
2005-06-01
影响因子：
10.3
通讯作者：
Fontao, L
中科院分区：
文献类型：
--
作者：
Lebeau, S;Masouyé, I;Fontao, L
背景ERBIN是Erb-B 2的结合伴侣，Erb-B 2是Erb-B家族中的孤儿受体，在细胞生长和分化的调节中起关键作用。ERBIN和Erb-B2在正常皮肤和皮肤癌组织中的分布及表达水平的比较研究目的探讨ERBIN和Erb-B2在正常皮肤和皮肤癌组织中的分布及表达水平的差异。应用免疫组织化学方法对12例鳞状细胞癌（SCC）和5例角化棘皮瘤（KA）石蜡包埋切片进行ERBIN和Erb-B2的免疫组化分析。它们主要定位于分化的角质形成细胞和外分泌腺导管细胞的质膜上，而它们弥漫性地定位于基底角质形成细胞的细胞质中。在SCC和KA中，ERBIN和Erb-B2的亚细胞分布保持不变，而在BCC中，这两种蛋白质从质膜重新分布到胞浆聚集体中。结论ERBIN在正常人皮肤中的亚细胞定位与Erb-B2相似，并随细胞分化而变化。基于我们的发现和Erb-B2的生物学活性，可以想象ERBIN和Erb-B2的表达或功能紊乱与基底细胞癌恶性表型的发展有关。
Background ERBIN is a binding partner of Erb-B2, an orphan receptor within the Erb-B family critically involved in the regulation of cell growth and differentiation. Although its function remains unclear, ERBIN is thought to affect the polarity of epithelial cells and cell growth via the Ras signalling pathway.Objectives To examine and compare the tissue distribution and the expression levels of ERBIN and Erb-B2 in normal skin and in cutaneous carcinomas.Methods Fifteen cases of basal cell carcinoma (BCC), 12 cases of squamous cell carcinoma (SCC) and five cases of keratoacanthoma (KA) were analysed by immunohistochemistry on paraffin-embedded sections using anti-ERBIN and anti-Erb-B2 antibodies.Results ERBIN and Erb-B2 had a similar distribution in normal human skin. They were primarily localized at the plasma membrane in differentiated keratinocytes and in duct cells from eccrine glands, whereas they were localized diffusely in the cytoplasma of basal keratinocytes. In both SCC and KA the subcellular distribution of ERBIN and Erb-B2 remained unchanged, whereas both proteins were redistributed from the plasma membrane into cytosolic aggregates in BCC.Conclusions The subcellular localization of ERBIN in normal human skin is similar to that of Erb-B2 and varies with cell differentiation. Based on our findings and on the biological activities of Erb-B2, it is conceivable that disturbed expression or functioning of ERBIN and Erb-B2 is implicated in the development of the malignant phenotype of BCC.