Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions.
Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions.
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sor 基因缺失的 HTLV-III/LAV 的复制和细胞病变潜力。
DOI：
10.1126/science.3006244
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Haseltine,W
中科院分区：
文献类型：
--
作者：
Sodroski,J;Goh,WC;Rosen,C;Tartar,A;Portetelle,D;Burny,A;Haseltine,W
人类嗜T淋巴细胞病毒III型(HTLV-III/LAV)的基因组除了编码gag、pol1和env基因编码的多肽外，还具有编码至少三种多肽的潜力。在本研究中，与该基因3‘端重叠的短开放阅读框(SOR)区的产物被发现是一种相对分子质量为23,000的蛋白质。建立了一种检测克隆的HTLV-III前病毒产生T4+淋巴细胞病变病毒能力的方法。在所使用的细胞系C8166中，HTLV-IIIsorgene产物和完整的3‘-orf基因产物都不是HTLV-III复制或细胞病变效应所必需的。
The genome of the human T-lymphotropic virus type III (HTLV-III/LAV) has the potential to encode at least three polypeptides in addition to those encoded by thegag,pol, andenvgenes. In this study, the product of the sor (short open reading frame) region, which overlaps the 3′ end of thepolgene, was found to be a protein with a molecular weight of 23,000. An assay was developed for testing the ability of cloned HTLV-III proviruses to produce viruses cytopathic for T4+lymphocytes. In the cell line used, C8166, neither the HTLV-IIIsorgene product nor the complete 3′-orfgene product were necessary for the replication or cytopathic effects of the HTLV-III.