Oxytocin is expressed in epidermal keratinocytes and released upon stimulation with adenosine 5'-[γ-thio]triphosphate in vitro
Oxytocin is expressed in epidermal keratinocytes and released upon stimulation with adenosine 5'-[γ-thio]triphosphate in vitro
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DOI：
10.1111/j.1600-0625.2012.01507.x
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发表时间：
2012-07-01
影响因子：
3.6
通讯作者：
Denda, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Denda, Sumiko;Takei, Kentaro;Denda, Mitsuhiro
催产素是一种主要在下丘脑产生的神经肽，以其在分娩和哺乳中的作用而闻名。它还影响行为、记忆和精神状态。最近的研究表明催产素在包括皮肤在内的外周组织中具有多种作用。在这里，我们展示了催产素在人类皮肤中表达。免疫组织化学研究表明，催产素及其载体蛋白神经素 I 主要位于表皮。 RT-PCR 证实了皮肤和培养的表皮角质形成细胞中催产素的表达。我们还表明，在应用 5'-[β-硫代]三磷酸腺苷（ATP?S，ATP 的稳定类似物）后，催产素以剂量依赖性方式从角质形成细胞中释放。 ATP?S诱导的催产素释放可通过去除细胞外钙或通过P2X受体拮抗剂2',3'-O-(2,4,6-三硝基苯基)腺苷5'-三磷酸(TNP-ATP)来抑制。这些结果表明催产素是在人表皮角质形成细胞中产生的，并通过 P2X 受体响应钙流入而释放。
Oxytocin is a neuropeptide produced primarily in the hypothalamus and is best known for its roles in parturition and lactation. It also influences behaviour, memory and mental state. Recent studies have suggested a variety of roles for oxytocin in peripheral tissues, including skin. Here we show that oxytocin is expressed in human skin. Immunohistochemical studies showed that oxytocin and its carrier protein, neurophysin I, are predominantly localized in epidermis. RT-PCR confirmed the expression of oxytocin in both skin and cultured epidermal keratinocytes. We also show that oxytocin is released from keratinocytes after application of adenosine 5'-[?-thio]triphosphate (ATP?S, a stable analogue of ATP) in a dose-dependent manner. The ATP?S-induced oxytocin release was inhibited by removal of extracellular calcium, or by the P2X receptor antagonist 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP). These results suggest that oxytocin is produced in human epidermal keratinocytes and is released in response to calcium influx via P2X receptors.