Genomic drivers of early-life fitness in Picea rubens
Genomic drivers of early-life fitness in Picea rubens
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DOI：
10.1007/s10592-021-01378-7
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发表时间：
2021-05-18
影响因子：
2.2
通讯作者：
Keller, Stephen R.
中科院分区：
文献类型：
--
作者：
Capblancq, Thibaut;Munson, Helena;Keller, Stephen R.
红云杉（Picea Rubens sarg。）是一棵针叶树，在北美北美蒙塔尼森林中有高度分散的范围。它是许多本地稀有和受威胁的分类单元的基础物种，因此是旨在恢复这些蒙塔尼生态系统的大规模重新造林工作的重点，但是缺乏指导这些努力的基因投入。为了解决这个问题，我们利用了一个常见的花园实验和整个外显子组测序数据集，以研究不同种群遗传参数对红云杉早期幼苗适应性的影响。评估了从物种范围的65个地方采样的340棵母亲的近交，遗传多样性和遗传负荷的水平，并将其与在受控环境中种植的5100个幼苗上测得的不同适应性特征进行了比较。我们确定了遗传多样性的总体积极影响以及遗传负荷和人口水平的负面影响对早期健康状况。这些关联对于阿巴拉契亚省中部和南部的高度分散的人群最为明显，在阿巴拉契亚人中，遗传多样性较低和较高的近交是与幼苗的发芽率较低，高度较短以及较低的高度和降低的早期生活适应性有关。这些结果提供了前所未有的信息，旨在恢复红云杉森林并最大化未来种植园的成功的现场经理可以使用。
Red spruce (Picea rubens Sarg.) is a coniferous tree with a highly fragmented range in eastern North American montane forests. It serves as a foundational species for many locally rare and threatened taxa and has therefore been the focus of large-scale reforestation efforts aimed at restoring these montane ecosystems, yet genetic input guiding these efforts has been lacking. To tackle this issue, we took advantage of a common garden experiment and a whole exome sequencing dataset to investigate the impact of different population genetic parameters on early-life seedling fitness in red spruce. The level of inbreeding, genetic diversity and genetic load were assessed for 340 mother trees sampled from 65 localities across the species range and compared to different fitness traits measured on 5100 of their seedlings grown in a controlled environment. We identified an overall positive influence of genetic diversity and negative impact of genetic load and population-level inbreeding on early-life fitness. Those associations were most apparent for the highly fragmented populations in the Central and Southern Appalachians, where lower genetic diversity and higher inbreeding were associated with lower germination rate, shorter height and reduced early-life fitness of the seedlings. These results provide unprecedented information that could be used by field managers aiming to restore red spruce forests and to maximize the success of future plantations.