Anaerobic biotransformation mechanism of marine toxin domoic acid
Anaerobic biotransformation mechanism of marine toxin domoic acid
复制标题
海洋毒素软骨藻酸的厌氧生物转化机制
DOI：
10.1016/j.jhazmat.2021.126798
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发表时间：
2021-08-06
影响因子：
13.6
通讯作者：
Gu, Chen
中科院分区：
文献类型：
--
作者：
Du, Miaomiao;Li, Zelong;Gu, Chen
Domoic acid (DA) is a major marine neurotoxin, occurs frequently in most of the world's coastlines and seriously threatens ecosystem and public health. However, information on its biotransformation process in coastal anaerobic environments remains unclear. In this study, the underlying mechanism of anaerobic biotransformation of DA by marine consortium GLY was investigated using the combination of liquid chromatography-highresolution Orbitrap mass spectrometry and comparative metatranscriptomics analysis. The results demonstrated that DA could be cometabolically biotransformed under anaerobic conditions with pseudo-first-order reaction. Anaerobic biotransformation pathway of DA was clarified, including decarboxylation, dehydrogenation, carboxylation activation with CoA and multiple 13-oxidation steps occurring at aliphatic side chain, which facilitated DA detoxification. Furthermore, anaerobic cometabolic biotransformation mechanism of glycine-DA by consortium GLY was established for the first time, a number of genes related to the metabolic pathways of glycine fermentation, fatty acid synthesis and 13-oxidation were responded in the consortium GLY transcriptome and involved in the anaerobic biotransformation of DA. This study could deepen understanding of interaction mechanism between toxin DA and marine microorganisms, which provides a new insight into the DA fate and its effects on benthic microbial community in marine environments.
