Effect of in vitro culture and cyclosporine A treatment on adrenal medulla allograft survival.
Effect of in vitro culture and cyclosporine A treatment on adrenal medulla allograft survival.
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体外培养和环孢素 A 处理对同种异体肾上腺髓质移植物存活的影响。
DOI：
10.1016/0022-4804(89)90042-5
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Lacy,PE
中科院分区：
文献类型：
--
作者：
Ricordi,C;Cryer,PE;Lacy,PE
将Wistar-Furth大鼠肾上腺髓质组织移植于刘易斯大鼠肾包膜下，观察供者移植物体外培养及受体环孢素A（CsA）免疫抑制对移植物存活的影响。肾上腺髓质在解剖后立即移植，或在24°C或在37°C的95%O2存在下培养1周后移植。结果表明，无论是文化的预处理影响移植后30天的移植物的形态完整性所示的同系移植物的存活。在没有供体骨髓体外培养和受体短期CsA治疗的情况下，所有同种异体移植物在30天时完全排斥。在24°C或95%O2存在下对移植物进行培养预处理，结合受体的暂时CsA治疗，或单独CsA治疗，移植后30天移植物的组织学存活。移植物中存在不同程度的淋巴细胞浸润。当肾上腺皮质与髓质组织一起移植时，观察到双峰免疫反应;髓质组织被淋巴细胞浸润，而肾上腺皮质在形态上保持完整，根本没有浸润。所进行的实验没有显示出使用移植前组织培养延长同种异体骨髓移植物存活的益处。
Adrenal medullary tissue from Wistar-Furth rats was transplanted beneath the renal capsule of Lewis rats to determine the effect ofin vitroculture of the donor graft and temporary recipient immunosuppression with Cyclosporine A (CsA) on allograft survival. The adrenal medulla was transplanted immediately after dissection or after 1 week of culture, either at 24°C or in the presence of 95% O2at 37°C. The results showed that neither cultural pretreatments affect the survival of the isografts as indicated by morphologic integrity of the grafts 30 days after transplantation. In the absence ofin vitroculture of the donor medulla and short-term CsA recipient treatment, all the allografts were completely rejected at 30 days. Cultural pretreatment of the grafts either at 24°C or in the presence of 95% O2, in conjunction with temporary CsA treatment of the recipient, or CsA treatment alone produced histologic survival of the grafts 30 days after transplantation. Varying degrees of lymphocytic infiltration were present in the grafts. When adrenal cortex was transplanted in conjunction with medullary tissue a bimodal immune reaction was observed; medullary tissue was infiltrated by lymphocytes, while the adrenal cortex remained morphologically intact with no infiltration at all. The experiments performed did not show a benefit in prolonging medulla allograft survival using pretransplant culture of the tissue.