Chemical disruption of yeast cells for the isolation of carotenoid pigments
Chemical disruption of yeast cells for the isolation of carotenoid pigments
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DOI：
10.1016/j.seppur.2006.06.026
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发表时间：
2007-02-25
影响因子：
8.6
通讯作者：
Chu, K. H.
中科院分区：
文献类型：
--
作者：
Park, P. K.;Kim, E. Y.;Chu, K. H.
红酵母红酵母产生有价值的类胡萝卜素色素，可用作天然食品着色剂和鱼类饲料中的色素来源。在这项工作中，培养并收获 R. glutinis，发酵 3 天后用于提取类胡萝卜素。使用五种不同的溶剂破坏冻干的 R. glutinis 细胞的细胞壁，从而释放细胞内产生的类胡萝卜素。色谱分析表明提取物中存在三种类胡萝卜素色素（β-胡萝卜素、Torulene 和 torularhodin）。采用 Box-Behnken 实验设计统计研究五种测试溶剂中的三种（二甲亚砜、石油醚和丙酮）和饱和氯化钠溶液对萃取程度的主要影响和交互影响。根据实验结果开发了一个将提取率与四个变量相关的经验模型。该模型被证明是对类胡萝卜素提取过程的统计上显着的描述，并且可以成功地用于导出四个变量的最有效组合，以从 R. glutinis 中提取类胡萝卜素。在最佳条件下进行的提取导致了类胡萝卜素的高产率，这表明组合的化学混合物可以有效地破坏 R. glutinis 细胞壁的完整性。 (c) 2006 Elsevier B.V. 保留所有权利。
The red yeast Rhodotorula glutinis produces valuable carotenoid pigments which are used as natural food colorants and as a pigment source in fish diets. In this work R. glutinis was cultivated and harvested for carotenoid extraction after 3 days of fermentation. Five different solvents were used to disrupt the cell wall of freeze-dried R. glutinis cells and thus release the intracellularly produced carotenoids. Chromatographic analysis showed that three carotenoid pigments (P-carotene, torulene, and torularhodin) were present in the extract. A Box-Behnken experimental design was employed to investigate statistically the main and interactive effects of three of the five solvents tested (dimethyl sulfoxide, petroleum ether, and acetone) and saturated NaCl solution on the extent of extraction. An empirical model relating extraction yield to the four variables was developed on the basis of the experimental results. The model was shown to be a statistically significant description of the carotenoid extraction process and could be successfully used to derive the most efficient combination of the four variables to extract carotenoids from R. glutinis. Extraction conducted under the optimum conditions resulted in a high yield of carotenoids, suggesting that the combined chemical cocktail was effective at compromising the integrity of the R. glutinis cell wall. (c) 2006 Elsevier B.V. All rights reserved.