Multiple JC virus genomes from one patient.
Multiple JC virus genomes from one patient.
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来自一名患者的多个 JC 病毒基因组。
DOI：
10.1099/0022-1317-65-8-1405
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Foster,GC
中科院分区：
文献类型：
--
作者：
Martin,JD;Foster,GC
JC病毒先前从一名进行性多灶性白质脑病患者的尿液和患病大脑中分离出来。这些分离物的DNA，MAD-7和MAD-8，分别显示在这项研究中是几个不同的全长基因组的混合物。根据其限制性内切酶切割模式的差异，将基因组分为两种类型。I型DNA被定义为以原型病毒MAD-1为代表的DNA; II型，以从MAD-7/8患者的脑组织分子克隆的病毒DNA（BrC-8 DNA）为代表。MAD-7 DNA是I型和II型的大致相等的混合物; MAD-8 DNA似乎是均匀的II型。这些DNA被分子克隆用于进一步的研究。在17个重组MAD-7（rMAD-7）DNA克隆中，9个为I型，彼此相同，并与MAD-1 DNA相同。其他8个克隆包括5个不同的II型物种，没有一个与MAD-8或BrC-8 DNA相同。在17个重组MAD-8（rMAD-8）克隆中，有两个含有I型DNA，这在原始病毒DNA中没有检测到。剩余的rMAD-8克隆为II型。所有这些DNA之间的差异主要是在“额外的”PvuII位点和在基因组的高变调控区（0.67至0.72图单位）的插入和缺失。多个基因组在不同的菌株从同一个病人进行了讨论。结论：观察到的变异起源于体内，而不是在体外分离。
JC virus was previously isolated from the urine and from diseased brain of a patient with progressive multifocal leukoencephalopathy. The DNAs of these isolates, MAD-7 and MAD-8 respectively, were shown in this study to be mixtures of several different full-length genomes. By differences in their restriction endonuclease cleavage patterns, the genomes were categorized into two types. Type I DNA was defined as that typified by the prototype virus, MAD-1; Type II, that typified by viral DNA molecularly cloned from brain tissue of the MAD-7/8 patient (BrC-8 DNA). MAD-7 DNA was an approximately equal mixture of Type I and Type II; MAD-8 DNA appeared to be homogeneously Type II. These DNAs were molecularly cloned for further studies. Of 17 clones of recombinant MAD-7 (rMAD-7) DNA, nine were Type I and were identical to each other and to MAD-1 DNA. The other eight clones comprised five different species of Type II, none of which was identical to MAD-8 or to BrC-8 DNA. Among 17 recombinant MAD-8 (rMAD-8) clones there were two which contained Type I DNA, which had not been detected in the original viral DNA. The remaining rMAD-8 clones were of Type II. The differences among all these DNAs were primarily in the ‘extra’PvuII sites and in the insertions and deletions in the hypervariable regulatory region of the genome (0.67 to 0.72 map unit). Possible explanations for multiple genomes in separate isolates from the same patient are discussed. It is concluded that the observed variation originatedin vivoand not during isolationin vitro.