Expression patterns of atrial natriuretic peptide and its receptors within the cochlear spiral ganglion of the postnatal rat
Expression patterns of atrial natriuretic peptide and its receptors within the cochlear spiral ganglion of the postnatal rat
复制标题
产后大鼠耳蜗螺旋神经节内心房钠肽及其受体的表达模式
DOI：
10.1016/j.heares.2013.11.010
复制
发表时间：
2014-03-01
期刊：
影响因子：
2.8
通讯作者：
Qiu, Jian-hua
中科院分区：
文献类型：
--
作者：
Sun, Fei;Zhou, Ke;Qiu, Jian-hua
螺旋神经节主要由螺旋神经节神经元和卫星神经胶质细胞组成，将听觉信息从感觉毛细胞传递到中枢神经系统。心钠素(ANP)是一种调节心脏、神经和神经胶质细胞功能的多肽，通过特定的受体发挥作用。虽然以往的研究已经为ANP及其功能性受体(NPR-A和NPR-C)在内耳中的存在提供了直接证据，但它们在耳蜗螺旋神经节中的存在及其在听神经传递和发育中的调节作用尚不清楚。本研究采用免疫荧光和免疫电子显微镜技术、逆转录-聚合酶链式反应和免疫印迹技术，研究了ANP及其受体在新生大鼠耳蜗螺旋神经节中的表达模式和水平。我们已经证明ANP及其受体共存于螺旋神经节神经元的两个亚型和神经节周围卫星神经胶质细胞。此外，我们还分析了与出生后发育过程中大鼠螺旋神经节内ANP及其受体的mRNA和蛋白的差异表达水平。总之，我们的研究为ANP及其受体的存在和合成提供了直接证据，提示ANP可能在听神经传递和发育过程中调节螺旋神经节内的神经元和神经胶质功能，以及神经元卫星神经胶质细胞通讯。(C)2013爱思唯尔B.V.保留所有权利。
The spiral ganglion, which is primarily composed of spiral ganglion neurons and satellite glial cells, transmits auditory information from sensory hair cells to the central nervous system. Atrial natriuretic peptide (ANP), acting through specific receptors, is a regulatory peptide required for a variety of cardiac, neuronal and glial functions. Although previous studies have provided direct evidence for the presence of ANP and its functional receptors (NPR-A and NPR-C) in the inner ear, their presence within the cochlear spiral ganglion and their regulatory roles during auditory neurotransmission and development is not known. Here we investigated the expression patterns and levels of ANP and its receptors within the cochlear spiral ganglion of the postnatal rat using immunofluorescence and immunoelectron microscopy techniques, reverse transcription-polymerase chain reaction and Western blot analysis. We have demonstrated that ANP and its receptors colocalize in both subtypes of spiral ganglion neurons and in perineuronal satellite glial cells. Furthermore, we have analyzed differential expression levels associated with both mRNA and protein of ANP and its receptors within the rat spiral ganglion during postnatal development. Collectively, our research provides direct evidence for the presence and synthesis of ANP and its receptors in both neuronal and non-neuronal cells within the cochlear spiral ganglion, suggesting possible roles for ANP in modulating neuronal and glial functions, as well as neuron satellite glial cell communication, within the spiral ganglion during auditory neurotransmission and development. (C) 2013 Elsevier B.V. All rights reserved.