THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE CROSSOSTOMA LACUSTRE MITOCHONDRIAL GENOME - CONSERVATION AND VARIATIONS AMONG VERTEBRATES
THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE CROSSOSTOMA LACUSTRE MITOCHONDRIAL GENOME - CONSERVATION AND VARIATIONS AMONG VERTEBRATES
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DOI：
10.1093/nar/20.18.4853
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发表时间：
1992-09-25
影响因子：
14.9
通讯作者：
HUANG, PC
中科院分区：
文献类型：
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作者：
TZENG, CS;HUI, CF;HUANG, PC
摘要本研究以台湾山区溪流中的淡水泥鳅横口鳅为研究对象，进行其线粒体全基因组的克隆与序列分析。该鱼mt基因组由16558个碱基对组成，编码13种蛋白质、2种rRNA和22种tRNA的基因，此外还有一个复制和转录的调控序列（D-loop），这些基因的顺序和方向与其他脊椎动物相似。蛋白质编码基因和核糖体RNA基因在大小和组成上与哺乳动物、鸟类、两栖动物和无脊椎动物中的对应物高度同源，并且使用基本上相同的密码子组，包括起始和终止信号以及tRNA。然而，D环区域的长度和序列以及基因之间的空间确实存在差异，这解释了基因组总长度的变化。我们的观察首次为鱼类线粒体基因组中遗传信息的保守性提供了证据，特别是在脊椎动物中。
The complete mitochondrial (mt) genome of Crossostoma lacustre, a freshwater loach from mountain stream of Taiwan, has been cloned and sequenced. This fish mt genome, consisting of 16558 base-pairs, encodes genes for 13 proteins, two rRNAs, and 22 tRNAs, in addition to a regulatory sequence for replication and transcription (D-loop), is similar to those of the other vertebrates in both the order and orientation of these genes. The protein-coding and ribosomal RNA genes are highly homologous both in size and composition, to their counterparts in mammals, birds, amphibians, and invertebrates, and using essentially the same set of codons, including both the initiation and termination signals, and the tRNAs. Differences do exist, however, in the lengths and sequences of the D-loop regions, and in space between genes, which account for the variations in total lengths of the genomes. Our observations provide evidence for the first time for the conservation of genetic information in the fish mitochondrial genome, especially among the vertebrates.