The fracture gap size influences the local vascularization and tissue differentiation in callus healing
The fracture gap size influences the local vascularization and tissue differentiation in callus healing
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DOI：
10.1007/s00423-003-0396-0
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发表时间：
2003-10-01
影响因子：
2.3
通讯作者：
Augat, P
中科院分区：
文献类型：
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作者：
Claes, L;Eckert-Hübner, K;Augat, P
背景骨折的血运重建取决于骨折稳定性和骨折间隙条件。本研究的目的是定量测定不同骨折间隙和受控生物力学条件下动物模型的血运重建和组织分化。材料和方法。对10只绵羊进行了研究，在右跖骨上进行了截骨术。骨折通过外固定器固定，允许可调节的轴向骨折块间运动。在可比的片段间应变（30-32%）下，将两组（每组5只羊）调整为中等大小的间隙（M，2.1 mm）和大间隙（L，5.7 mm）。9周后处死动物，制备跖骨用于不脱钙组织学和组织分化和血管分布的分析。结果与L组相比，M组血管重建（M=1.62，L=0.85血管/mm（2））、骨形成（M= 37.2%，L=13.9%）和纤维软骨组织（M= 18.1%，L=39.1%）明显更多。大血管（>40 μ m）主要位于髓管，小血管（
Background. Revascularization of a fracture depends on fracture stability and fracture gap conditions. The aim of the study was to determine quantitatively the revascularization and tissue differentiation in an animal model with different fracture gaps and controlled biomechanical conditions. Materials and method. The study was performed on ten sheep with an osteotomy on the right metatarsal. The fracture was stabilized by an external fixator that allowed adjustable axial interfragmentary movement. Two groups of five sheep each were adjusted to a medium sized gap (M, 2.1 mm) and a large gap (L, 5.7 mm) under comparable interfragmentary strain (30-32%). The animals were killed after 9 weeks, and the metatarsals were prepared for undecalcified histology and analysis of tissue differentiation and vessel distribution. Results. Group M showed significantly more revascularization (M=1.62, L=0.85 vessels/mm(2)), more bone formation (M=37.2%, L=13.9%) and less fibrocartilage tissue (M=18.1%, L=39.1%) than group L. Larger vessels (>40 mum) were found mainly in the medullary channel, and smaller vessels (