THE MITOCHONDRIAL OUTER-MEMBRANE PROTEIN MAS22P IS ESSENTIAL FOR PROTEIN IMPORT AND VIABILITY OF YEAST
THE MITOCHONDRIAL OUTER-MEMBRANE PROTEIN MAS22P IS ESSENTIAL FOR PROTEIN IMPORT AND VIABILITY OF YEAST
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DOI：
10.1073/pnas.91.25.11973
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发表时间：
1994-12-06
影响因子：
11.1
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
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作者：
LITHGOW, T;JUNNE, T;SCHATZ, G
我们已经克隆了编码蛋白质Mas22p的基因，该蛋白质跨越酵母线粒体的外膜。完全缺乏Mas22p的细胞是不可存活的。质粒携带的MAS22基因抑制由一个或多个线粒体蛋白输入受体缺失引起的几种缺陷。Mas20 p缺陷型细胞的缺陷可以通过这些突变体中Mas22 p水平的降低来解释。Mas22p在细胞质中有一个酸性结构域，在线粒体膜间隙中有第二个酸性结构域。我们认为Mas22p外膜两侧的这些结构域作为一个中继系统，将前体蛋白的基本靶向序列转移到线粒体中。
We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses several defects resulting from the deletion of one or more of the mitochondrial protein import receptors. Defects of Mas20p-deficient cells are explained by the reduced level of Mas22p in these mutants. Mas22p has one acidic domain in the cytosol and a second acidic domain in the mitochondrial intermembrane space. We suggest that these domains of Mas22p on either side of the outer membrane function as a relay system for transferring the basic targeting sequences of precursor proteins into the mitochondria.