High-resolution multitransistor array recording of electrical field potentials in cultured brain slices
High-resolution multitransistor array recording of electrical field potentials in cultured brain slices
复制标题
DOI：
10.1152/jn.00347.2006
复制
发表时间：
2006-09-01
影响因子：
2.5
通讯作者：
Fromherz, P.
中科院分区：
文献类型：
--
作者：
Hutzler, M.;Lambacher, A.;Fromherz, P.
我们报道了用16,384个元件的多晶体管阵列(MTA)记录培养的海马片的电活动。在2 kHz下，在1 mm(2)的面积上实现了7.8µm的时间分辨成像。较少元素的读出允许增强的时间分辨率。单个晶体管信号是由局部激发场电位引起的。它们在幅度和形状上与微吸管的测量结果一致。记录的空间连续性提供了诱发场电位的时间分辨图像，并允许检测远距离的功能相关性。作为例子，记录了突触前动作电位的快速传播波，以及兴奋性突触后电位横跨和沿着腺角的模式。
We report on the recording of electrical activity in cultured hippocampal slices by a multitransistor array (MTA) with 16,384 elements. Time-resolved imaging is achieved with a resolution of 7.8 mu m on an area of 1 mm(2) at 2 kHz. A read-out of fewer elements allows an enhanced time resolution. Individual transistor signals are caused by local evoked field potentials. They agree with micropipette measurements in amplitude and shape. The spatial continuity of the records provides time-resolved images of evoked field potentials and allows the detection of functional correlations over large distances. As examples, fast propagating waves of presynaptic action potentials are recorded as well as patterns of excitatory postsynaptic potentials across and along cornu ammonis.