Comparative Transcriptome Analysis of Gene Expression and Regulatory Characteristics Associated with Different Vernalization Periods in Brassica rapa
Comparative Transcriptome Analysis of Gene Expression and Regulatory Characteristics Associated with Different Vernalization Periods in Brassica rapa
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DOI：
10.3390/genes11040392
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发表时间：
2020-04-01
期刊：
影响因子：
3.5
通讯作者：
Sun, Rifei
中科院分区：
文献类型：
--
作者：
Dai, Yun;Zhang, Shujiang;Sun, Rifei
芜菁是我国重要的蔬菜作物，对人体健康有益。影响B的主要因素。芜菁产量是低温的，这促进了抽苔和开花，从而降低了其商业价值。然而，加速抽苔和开花可用于快速育种。因此，研究B春化的分子机制是十分必要的。《行动计划》对于解决与生产有关的问题至关重要。这是两个B的转录组。对不同春化时期的芜菁材料进行了综合分析。在春化过程中，获得了总共974，584，022个干净读段和291.28 Gb的干净数据。与B的参考基因组相比。在rapa基因组中，共鉴定出44,799个已知基因和2280个新基因。采用自组织特征图谱分析方法对两个B中的21,035个差异表达基因进行筛选。rapa accessions，'Jin Wawa' and 'Xiao Baojian'.分析表明，在春化过程中，与植物激素信号转导、淀粉和蔗糖代谢、光周期和生物钟以及春化途径相关的转录本发生了显著变化。此外，TPS、UGP、CDF、VIN 1等7个激素途径基因在春化过程中的表达模式也存在差异。本研究的转录组结果为B期间发生的变化提供了新的视角。芜菁春化作用，以及作为一个很好的参考B。芜菁育种
Brassica rapa is an important Chinese vegetable crop that is beneficial to human health. The primary factor affecting B. rapa yield is low temperature, which promotes bolting and flowering, thereby lowering its commercial value. However, quickened bolting and flowering can be used for rapid breeding. Therefore, studying the underlying molecular mechanism of vernalization in B. rapa is crucial for solving production-related problems. Here, the transcriptome of two B. rapa accessions were comprehensively analyzed during different vernalization periods. During vernalization, a total of 974,584,022 clean reads and 291.28 Gb of clean data were obtained. Compared to the reference genome of B. rapa, 44,799 known genes and 2280 new genes were identified. A self-organizing feature map analysis of 21,035 differentially expressed genes was screened in two B. rapa accessions, 'Jin Wawa' and 'Xiao Baojian'. The analysis indicated that transcripts related to the plant hormone signal transduction, starch and sucrose metabolism, photoperiod and circadian clock, and vernalization pathways changed notably at different vernalization periods. Moreover, different expression patterns of TPS, UGP, CDF, VIN1, and seven hormone pathway genes were observed during vernalization between the two accessions. The transcriptome results of this study provide a new perspective on the changes that occur during B. rapa vernalization, as well as serve as an excellent reference for B. rapa breeding.