Endothelial Differentiated Adipose-Derived Stem Cells Improvement of Survival and Neovascularization in Fat Transplantation
Endothelial Differentiated Adipose-Derived Stem Cells Improvement of Survival and Neovascularization in Fat Transplantation
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DOI：
10.1093/asj/sjy130
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发表时间：
2019-01-17
影响因子：
2.9
通讯作者：
Zhang, Ping
中科院分区：
文献类型：
--
作者：
Harris, William M.;Plastini, Michael;Zhang, Ping
背景 脂肪干细胞（ASC）辅助脂肪移植被认为可以促进脂肪移植物的存活。然而，新出现的血管化不足的证据是细胞辅助脂肪移植中脂肪移植物存活的另一个障碍。目的 本研究评估脂肪抽吸术中的内皮表型 ASC 能否改善脂肪移植中的存活率和新血管形成。 方法 从人脐周脂肪组织中分离出 ASC，并在内皮生长培养基中培养 2 周。将脂肪抽吸物与新鲜脂肪基质血管部分（SVF）、内皮分化的ASC（EC/ASC）以及单独的脂肪抽吸物混合。将三种脂肪混合物皮下注射到成年雄性Sprague-Dawley大鼠背部的3个位置。移植后8周，收获移植的脂肪抽吸物，并使用照相、存活体重测量和组织学检查来评估提取的脂肪。通过免疫荧光和实时 RT-PCR 对新血管形成进行定量。结果 EC/ASC辅助组的移植物具有较高的成活率、形态完整性和最均匀的脂滴。通过组织学和免疫荧光分析，他们还发现炎症和纤维化减少，血管数量增加。定量RT-PCR分析表明，与对照组和SVF移植脂肪组相比，EC/ASC组中CD31和vWF的EC特异性标志物的表达水平较高。结论 这些结果表明，脂肪抽吸物与分化为内皮表型的 ASC 共同植入可提高移植脂肪抽吸物的存活率和新血管形成，这可能会带来益处，并代表自体脂肪移植临床应用的一种有前途的策略。
Background Adipose-derived stem cells (ASCs) assisted lipotransfer have been considered to facilitate the survival of fat grafts. However, emerging evidence of insufficient vascularization is another obstacle for fat graft survival in cell-assisted lipotransfer. Objectives This study evaluated if endothelial phenotype ASCs with fat lipoaspirate improves survival and neovascularization in fat transplantation Methods ASCs were isolated from human periumbilical fat tissue and cultured in endothelial growth medium for 2 weeks. Fat lipoaspirate was mixed with fresh adipose stroma vascular fraction (SVF), endothelial differentiated ASCs (EC/ASCs), and fat lipoaspirate alone. Three fat mixtures were subcutaneously injected into the adult male Sprague-Dawley rat's dorsum at 3 locations. At 8 weeks after transplantation, the grafted fat lipoaspirates were harvested, and the extracted fat was evaluated using photographic, survival weights measurements and histological examination. Neo-vascularization was quantified by immunofluorescence and real-time RT-PCR. Results Grafts from the EC/ASC assisted group had a higher survival rate, morphologic integrity, and most uniform lipid droplets. They also revealed less inflammation and fibrosis with increased number of vessels by histological and immunofluorescence analysis. Quantitative RT-PCR analysis indicated that the expression levels of EC-specific markers of CD31 and vWF were higher in the EC/ASC group compared with in the control and fat with SVF transplants. Conclusions These results indicated that co-implantation of fat lipoaspirate with ASCs differentiated toward an endothelial phenotype improves both survival and neovascularization of the transplanted fat lipoaspirate, which might provide benefits and represents a promising strategy for clinical application in autologous fat transplantation.