Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.
Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.
复制标题
编码潜在 Epstein-Barr 病毒核抗原 I 的转录物分析：由几个外显子的长程剪接产生的潜在多顺反子信息。
DOI：
10.1073/pnas.82.24.8305
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发表时间：
1985
影响因子：
11.1
通讯作者：
Strominger,JL
中科院分区：
文献类型：
--
作者：
Speck,SH;Strominger,JL
潜伏感染细胞中存在的EB病毒核抗原（EBNA I）编码在BamHI K病毒基因组片段中含有的2-乙酰氨基转移酶外显子中。然而，该外显子存在于3.7-腺苷三磷酸酶mRNA转录物中。其余的1.7种酶的来源是未知的，尽管它不是来自相邻的EB病毒DNA序列。用与BamH Ⅰ K片段中EBNA Ⅰ假定起始密码子3 ′端245个碱基对序列互补的寡核苷酸，通过引物延伸产生1.1-腺苷酸酶cDNA克隆。该克隆包含7个外显子（来自BamHI W、Y、U、E和K病毒基因组片段），其分布在病毒基因组的约70个内切酶上。然而，该克隆似乎不包含转录本的完整5'末端。除了编码EBNA I的外显子中的开放阅读框外，在该转录物中发现了三个其他开放阅读框，其可能编码潜伏感染细胞中存在的其他病毒抗原。
The Epstein-Barr virus nuclear antigen (EBNA I) present in latently infected cells is encoded in a 2-kilobase exon contained in the BamHI K viral genomic fragment. This exon is, however, found within a 3.7-kilobase mRNA transcript. The origin of the remaining 1.7 kilobases is unknown, although it is not derived from adjacent Epstein-Barr virus DNA sequences. A 1.1-kilobase cDNA clone generated by primer extension using an oligonucleotide complimentary to a sequence 245 base pairs 3' to the putative initiation codon for EBNA I in the BamHI K fragment has been isolated. This clone contains seven exons (from the BamHI W, Y, U, E, and K viral genomic fragments), which are spread over approximately 70 kilobases of the viral genome. However, this clone does not appear to contain the complete 5' end of the transcript. In addition to the open reading frame in the exon encoding EBNA I, three other open reading frames are found in this transcript that potentially encode other viral antigens present in latently infected cells.