"Density Equilibrium" Method for the Quantitative and Rapid In Situ Determination of Lipid, Hydrocarbon, or Biopolymer Content in Microorganisms
"Density Equilibrium" Method for the Quantitative and Rapid In Situ Determination of Lipid, Hydrocarbon, or Biopolymer Content in Microorganisms
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DOI：
10.1002/bit.22182
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发表时间：
2009-04-01
影响因子：
3.8
通讯作者：
Melis, Anastasios
中科院分区：
文献类型：
--
作者：
Eroglu, Ela;Melis, Anastasios
这项工作提供了一个简单的方法，直接密度平衡测量的基础上，在各种原核和真核样品中的总脂质，烃或生物聚合物含量的快速原位估计。该方法可以容易地应用于活的微藻和光合细菌、单细胞或菌落微生物，以及细胞级分和分离的亚细胞区室或组分。在这种方法中，可以容易地计算细胞的绝对脂质、烃或生物聚合物含量。该方法尤其可用于跟踪微藻和其它微生物的菌株的油或聚合物含量，其脂质、烃或生物聚合物含量可随培养条件和/或时间而变化，如在微生物脂质诱导工业过程中的情况。该方法还可用于直接原位测量活细胞中的储存聚合物积累，例如光合细菌和非光合细菌中的微藻中的淀粉和聚羟基丁酸酯或其他聚羟基链烷酸酯。
The work provides a simple method, based on a direct density equilibrium measurement, for the rapid in situ estimation of total lipid, hydrocarbon or biopolymer content in a variety of prokaryotic and eukaryotic samples. The method can be readily applied to live microalgae and photosynthetic bacteria, single-celled or colonial microorganisms, as well as cellular fractions and isolated subcellular compartments or components. In this approach, the absolute lipid, hydrocarbon, or biopolymer content of the cells can be readily calculated. This method is especially useful for tracking the oil or polymer content of strains of microalgae and other microorganisms, whose lipid, hydrocarbon or biopolymer content may change with cultivation conditions and/or time, as the case would be in microorganism lipid-induction industrial processes. The method is also useful for the direct in Situ measurement of storage polymer accumulation in live cells, such as starch in microalgae and polyhydroxybutyrate, or other polyhydroxyalkanoates, in photosynthetic and non-photosynthetic bacteria.