In situ localization of agouti signal protein in murine skin using immunohistochemistry with an ASP-specific antibody.
In situ localization of agouti signal protein in murine skin using immunohistochemistry with an ASP-specific antibody.
复制标题
使用 ASP 特异性抗体进行免疫组织化学，对小鼠皮肤中刺鼠信号蛋白进行原位定位。
DOI：
10.1006/bbrc.2000.2409
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发表时间：
2000
影响因子：
3.1
通讯作者：
Hearing,VJ
中科院分区：
文献类型：
--
作者：
Matsunaga,N;Virador,V;Santis,C;Vieira,WD;Furumura,M;Matsunaga,J;Kobayashi,N;Hearing,VJ
在哺乳动物中，毛囊黑素细胞中真黑素或褐黑素的生产之间的转换是负责毛发颜色的;在小鼠中，这种转换由agglutinin基因座控制，该基因座通过黑皮质素受体1的作用编码agglutinin信号蛋白（ASP）。为了研究ASP在皮肤中的表达和加工模式及其对毛囊中色素产生的调节，我们已经产生了针对与ASP的羧基末端相对应的合成肽的兔抗体（称为α PEP 16）。通过ELISA测量该抗体的特异性，并通过Western印迹分析确认。使用免疫组化，我们的特点是ASP的表达在新生小鼠的皮肤在出生后3，6，9天。正如预期的那样，在所有检查的时间点，在非凝集素（a/a）黑色小鼠皮肤中的表达均为阴性，并且在6天新生凝集素（A/+）和6和9天新生致死黄色（Ay/a）小鼠皮肤中观察到ASP的高表达。在致死性黄（pheomelanogenic）小鼠中，ASP表达随着毛发颜色变得更黄而逐日增加。这些表达模式表明，ASP被快速有效地递送到黑素细胞和毛球中的毛基质细胞，在那里它调节黑素的产生。
Switching between production of eumelanin or pheomelanin in follicular melanocytes is responsible for hair color in mammals; in mice, this switch is controlled by the agouti locus, which encodes agouti signal protein (ASP) through the action of melanocortin receptor 1. To study expression and processing patterns of ASP in the skin and its regulation of pigment production in hair follicles, we have generated a rabbit antibody (termed αPEP16) against a synthetic peptide that corresponds to the carboxyl terminus of ASP. The specificity of that antibody was measured by ELISA and was confirmed by Western blot analysis. Using immunohistochemistry, we characterized the expression of ASP in the skin of newborn mice at 3, 6, and 9 days postnatally. Expression in nonagouti (a/a) black mouse skin was negative at all times examined, as expected, and high expression of ASP was observed in 6 day newborn agouti (A/+) and in 6 and 9 day newborn lethal yellow (Ay/a) mouse skin. In lethal yellow (pheomelanogenic) mice, ASP expression increased day by day as the hair color became more yellow. These expression patterns suggest that ASP is delivered quickly and efficiently to melanocytes and to hair matrix cells in the hair bulbs where it regulates melanin production.