Genomic analysis of Saccharomyces cerevisiae isolates that grow optimally with glucose as the sole carbon source.
Genomic analysis of Saccharomyces cerevisiae isolates that grow optimally with glucose as the sole carbon source.
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DOI：
10.1002/elps.201200172
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发表时间：
2012-12
期刊：
影响因子：
2.9
通讯作者：
Edwards, Jeremy S.
中科院分区：
文献类型：
--
作者：
Aragon, Anthony D.;Torrez-Martinez, Norah;Edwards, Jeremy S.
A population of Saccharomyces cerevisiae was cultured for approximately 450 generations in the presence of high glucose to select for genetic variants that grew optimally under these conditions. Using the parental strain BY4741 as the starting population, an evolved culture was obtained after aerobic growth in a high glucose medium for approximately 450 generations. After the evolution period three single colony isolates were selected for analysis. Next-generation Ion Torrent sequencing was used to evaluate genetic changes. Greater than 100 deletion/insertion changes were found with approximately half of these effecting genes. Additionally, over 180 single-nucleotide polymorphisms (SNPs) were identified with more than one quarter of these resulting in a non-synonymous mutation. Affymetrix DNA microarrays and RNseq analysis were used to determine differences in gene expression in the evolved strains compared to the parental strain. It was established that approximately 900 genes demonstrated significantly altered expression in the evolved strains relative to the parental strain. Many of these genes showed similar alterations in their expression in all three evolved strains. Interestingly, genes with altered expression in the three evolved strains included genes with a role in oxidative metabolism. Overall these results are consistent with the physiological observations of optimal growth with glucose as the carbon source. Namely, the decreased ethanol production suggest, that the underlying metabolism switched from fermentation to respiration during the selection for optimal growth on glucose.
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影响因子：
30.8
作者：
Deutscher, David;Meilijson, Isaac;Ruppin, Eytan
通讯作者：
Ruppin, Eytan
DOI：
10.1046/j.1432-1327.1998.2580736.x
发表时间：
1998-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Przybyla-Zawislak, B;Dennis, RA;McCammon, MT
通讯作者：
McCammon, MT
影响因子：
11.4
作者：
Piskur, J;Rozpedowska, E;Compagno, C
通讯作者：
Compagno, C
影响因子：
--
作者：
Vargas FA;Pizarro F;Pérez-Correa JR;Agosin E
通讯作者：
Agosin E
影响因子：
2.9
作者：
Cakir, Tunahan;Efe, Cagri;Oliver, Stephen G.
通讯作者：
Oliver, Stephen G.